Mapping of genes encoding glycoside hydrolases on the chromosome of Cellulomonas fimi

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Abstract:

Cellulomonas fimi genomic DNA was digested with HpaI, MunI, HindIII, and NsiI, producing fragments ranging in size from 20 to 1400 kbp that were resolved by pulsed field gel electrophoresis. Genetic and physical linkages were determined by Southern blotting and were used to construct a genome map. Cellulomonas fimi has a single circular chromosome of approx. 4000 kbp. Except for two closely linked genes, cbh6A and cel5A, the genes known to encode glycoside hydrolases are scattered widely on the chromosome.Key words: Cellulomonas fimi, genome map, pulsed field gel electrophoresis, glycoside hydrolases.

L'ADN génomique du Cellulomonas fimi a été digéré avec les enzymes HpaI, MunI, HindIII et NsiI produisant ainsi des fragments dont la taille variait de 20 à 1400 kpb qui ont été séparés par électrophorèse sur gel en champ pulsé. Les liens entre les caractéristiques physiques et génétiques ont été établis par buvardage Southern et utilisés pour construire une carte génomique. Cellulomonas fimi possède un seul chromosome circulaire d'environ 4000 kpb. À l'exception de deux gènes étroitement liés, cbh6A et cel5A, les gènes reconnus pour coder les glycoside hydrolases sont dispersés dans l'ensemble du chromosome.Mots clés : Cellulomonas fimi, carte génomique, électrophorèse sur gel en champ pulsé, glycoside hydrolases.[Traduit par la Rédaction]
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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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