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Selective isolation and study on the global distribution of the genus Planobispora in soils

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Planobispora strains, including the type strain, produced abundant sporangia on humic acid – vitamin gellan gum medium when more than 50 colonies grew on a plate. However, these strains did not produce sporangia on the aerial mycelium, a diagnostic characteristic of this genus, when fewer than 20 colonies grew on a plate. Trace salts, such as FeSO4·7H2O, MnCl2·4H2O, ZnSO4·7H2O, and NiSO4·6H2O, stimulated sporangium formation of Planobispora strains. Better sporangium formation of Planobispora strains was observed on the humic acid – trace salts gellan gum medium at pH 9.0 than at neutral pH. Moreover, an alkaline medium repressed the growth of three out of six Streptomyces strains so that this condition was effective for selective isolation of Planobispora strains. An alkaline flooding solution of skim milk, five antimicrobial agents, and dry heat treatment at 90°C for 60 min were effective for selective isolation. Using these techniques, we examined the distribution of Planobispora strains by using 1467 soil samples collected from Japan and other parts of the world. One hundred and nineteen Planobispora strains were isolated from 51 soil samples (3.5% of the samples tested) that were collected in Ecuador, Egypt, French Guiana, India, and Madagascar. Planobispora strains were recovered only in tropical and subtropical soils. To our knowledge, this is the first record that Planobispora strains have been isolated from locations other than Venezuela, Namibia, or South Africa.Key words: Planobispora, gellan gum, selective isolation, actinomycete, distribution.

Les souches de Planobispora incluant la souche type produisent une abondance de sporanges sur le milieu acide humique – vitamines et gomme gélifiante lorsque plus de 50 colonies poussaient sur une gélose. Toutefois, ces souches n'ont pas produit de sporange sur le mycélium aérien, une caractéristique propre à ce genre, lorsque moins de 20 colonies avaient poussé sur gélose. Des traces de sels tel que FeSO4·7H2O, MnCl2·4H2O, ZnSO4·7H2O et NiSO4·6H2O ont stimulé la formation de sporanges chez les souches de Planobispora. Une meilleure formation de sporanges a été observée sur le milieu acide humique – traces salines et gomme gélifiante à un pH de 9,0 qu'à un pH neutre. De plus, un milieu alcalin a inhibé la croissance de trois souches de Streptomyces sur six de telle façon que cette condition fut propice à l'isolation sélective de souches de Planobispora. Une solution d'immersion alcaline à base de lait écrémé, cinq agents anti-microbiens et une traitement thermique à sec à 90°C pendant 60 min ont permis une isolation sélective efficaces. En employant ces techniques, nous avons étudié la distribution de souches de Planobispora en utilisant 1467 échantillons de sol recueillis du Japon et d'autres parties du monde. Cent dix-neuf souches de Planobispora ont été isolées à partir de 51 échantillons de sol (3,5 % des échantillons analysés) qui provenaient de l'Équateur, l'Égypte, la Guyane française, l'Inde et Madagascar. Les souches de Planobispora ont seulement été retrouvées dans des sols tropicaux et sub-tropicaux. À notre connaissance, ceci est le premier signalement de souches de Planobispora ayant été isolées à partir d'endroits autres que le Venezuela, la Namibie ou l'Afrique du Sud.Mots clés : Planobispora, gomme gélifiante, isolation sélective, actinomycète, distribution.[Traduit par la Rédaction]

Keywords: Planobispora; actinomycete; actinomycète; distribution; gellan gum; gomme gélifiante; isolation sélective; selective isolation

Document Type: Research Article

Publication date: November 1, 2001

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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