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Phenotypic and genotypic characterization of esterase-producing Ureibacillus thermosphaericus isolated from an aerobic digestor of swine waste

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Abstract:

Eight closely related thermophilic strains were isolated from an aerobic and thermophilic treatment of swine wastes. The pleomorphic cells (short and long rods; cocci) showed peritrichous flagella, terminally swollen sporangium, and liberated spores exhibiting hairy appendages. The Gram reaction was negative for both young (4 h) and old (48 h) cultures. Several features, such as colonial morphology, growth between 35°C and 65°C, presence of catalase, presence of spores, and strictly aerobic metabolism (except for one strain), are similar to those found for the genus Bacillus. The inability of the strains to use sugars, except esculin, as source of carbon and energy and the whole cell fatty acid composition are similar to those found in Bacillus thermosphaericus DSM 10633. Sequence analysis of the 16S rRNA gene revealed 99.8%–99.9% identity for seven of the thermophilic strains with this species. A new genus, Ureibacillus, was recently proposed for type strain B. thermosphaericus DSM 10633 The last strain exhibits 97.8% and 97.3% identity with Ureibacillus terrenus DSM12654 and Bacillus sp. TP-84, respectively. Esterase activities were detected for all strains, and assays on p-nitrophenyl butyrate and p-nitrophenyl caprylate revealed that strains were more active on the shorter substrate.Key words: Ureibacillus thermosphaericus, thermophilic, esterase.

Huit souches apparentées ont été isolées d'un traitement de digestion aérobie du lisier de porc. Les souches prennent une coloration de type Gram négative, autant chez les jeunes cultures (4 h) que chez celles plus âgées (48 h). L'observation en microscopie électronique a permis d'observer des cellules pléomorphes (bâtonnets et coques de dimensions variables) présentant des flagelles péritriches, des spores terminales déformantes qui une fois libérées arborent des structures semblables à des poils. Plusieurs caractéristiques telles la morphologie coloniale, la température de croissance entre 35°C et 65°C, la présence de l'enzyme catalase, la présence de spores ainsi que le métabolisme aérobie strict (sauf une souche) étaient similaires à ce qui est retrouvé chez le genre Bacillus. L'incapacité des huit souches à utiliser les sucres (excepté l'esculine) comme source de carbone et d'énergie, ainsi que la composition de leurs acides gras cellulaires les associent étroitement à l'espèce Bacillus thermosphaericus DSM 10633. L'analyse des séquences du gène codant pour l'ARNr 16S a révélé une homologie de 99,8 % – 99,9 % de sept des souches thermophiles avec cette espèce. Un nouveau genre, Ureibacillus, a récemment été proposé pour la souche B. thermosphaericus DSM 10633. La huitième souche présentait une similitude de 97,8 % et 97,3 % avec les souches Ureibacillus terrenus DSM 12654 et Bacillus sp. TP-84, respectivement. Une activité d'hydrolyse sur la tributyrine et sur les esters de p-nitrophényl butyrate et de p-nitrophényl caprylate a été observée chez les huit souches.Mots clés : Ureibacillus thermosphaericus, thermophile, estérase.

Keywords: Ureibacillus thermosphaericus; esterase; estérase; thermophile; thermophilic

Document Type: Research Article

Publication date: October 1, 2001

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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