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Cloning and sequencing of two genes encoding chitinases A and B from Bacillus cereus CH

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Two genes encoding chitinases A and B (chiA and chiB) from Bacillus cereus CH were cloned into Escherichia coli XL1-Blue MRF' by using pBluescript II SK+, and their nucleotide sequences were determined. Open reading frames of the chiA and chiB genes encoded distinct polypeptide chains consisting of 360 and 674 amino acid residues, respectively, with calculated molecular sizes of 39 470 and 74 261 Da, respectively. Comparison of the deduced amino acid sequences with those of other bacterial chitinases revealed that chitinase A consisted of a catalytic domain, while chitinase B consisted of three functional domains, a catalytic domain, a fibronectin type III-like domain, and a cellulose-binding domain. The primary structures of these two proteins were not similar to each other.Key words: Bacillus cereus, chitinase, cloning.

Deux gènes codant pour les chitinases A et B (chiA et chiB) de Bacillus cereus CH ont été clonés dans Escherichia coli XL1-Blue MRF' en utilisant pBluescript II SK+, et leur séquence de nucléotides a été déterminée. Les cadres de lectures des gènes chiA et chiB codaient des polypeptides distincts de 360 et 674 acides aminés, respectivement, ayant des poids moléculaires calculés de 39 470 et 74 261 Da, respectivement. Une comparaison de la séquence déduite en acide aminés avec celles d'autres chitinases bactériennes a montré que la chitinase A était composée d'un domaine catalytique, alors que la chitinase B comportait trois domaines fonctionnels, un domaine catalytique, un domaine similaire à la fibronectine de type III et un domaine de liaison au cellulose. Les structures primaires de ces deux protéines de se ressemblaient pas.Mots clés : Bacillus cereus, chitinase, clonage.[Traduit par la Rédaction]

Keywords: Bacillus cereus; chitinase; clonage; cloning

Document Type: Research Article

Publication date: 2001-10-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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