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Reduction of linoleic acid inhibition in production of conjugated linoleic acid by Propionibacterium freudenreichii ssp. shermanii

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Abstract:

A method for the production of conjugated linoleic acid (CLA) from linoleic acid (LA) using growing cultures of Propionibacterium freudenreichii ssp. shermanii JS was developed. The growth inhibitory effect of LA was eliminated by dispersing it in a sufficient concentration of polyoxyethylene sorbitan monooleate detergent. For the whey permeate medium used, the optimum LA:detergent ratio was 1:15 (w/w). As a result, the cultures tolerated at least 1000 µg·mL–1 LA, which was converted to CLA with 57%–87% efficiency. The cis-9, trans-11 and trans-9, cis-11 isomers constituted 85%–90% of the CLA produced. The feasibility of the method was demonstrated also in de Man – Rogosa–Sharpe (MRS) broth.Key words: conjugated linoleic acid, linoleic acid, Propionibacterium freudenreichii ssp. shermanii.

Nous avons mis au point une méthode de production d'un conjugué d'acide linoléique (CLA) à partir de l'acide linoléique (LA) en utilisant des cultures actives de la souche JS de Propionibacterium freudenreichii ssp. shermanii. L'effet inhibiteur de LA sur la croissance fut être éliminé en dispersant cet acide dans une concentration suffisante d'un détergent, soit le polyoxyéthylène sorbitan monooléate. Avec le milieu au lactosérum, le rapport optimal LA:détergent était de 1:15 (p/p). De cette façon, les cultures toléraient au moins 1000 µg·mL–1 de LA qui se convertissait en CLA avec une efficacité de 57 % – 87 %. Les isomères cis-9, trans-11 et trans-9, cis-11 représentaient 85 % – 90 % du CLA produit. La faisabilité de la méthode a aussi été démontrée en utilisant le bouillon de Man – Rogosa–Sharpe (MRS).Mots clés : conjugué d'acide linoléique, acide linoléique, Propionibacterium freundenreichii ssp. shermanii.[Traduit par la Rédaction]

Keywords: Propionibacterium freudenreichii ssp; Propionibacterium freundenreichii ssp; acide linoléique; conjugated linoleic acid; conjugué; d'acide linoléique; linoleic acid

Document Type: Research Article

Publication date: 2001-08-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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