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Increased phospholipid transfer protein activity in Aspergillus oryzae grown on various industrial phospholipid sources

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The effect of industrial carbon sources on phospholipid transfer protein production was investigated. Phospholipid fractions of different composition were prepared from various plant oils (i.e., soybean, rapeseed, and sunflower) according to the Lucas Meyer extraction and purification process. The effect of these fractions on phospholipid transfer protein activity of cell extracts from Aspergillus oryzae grown on medium containing these phospholipids as sole carbon source was studied. It was shown that phospholipid transfer activity was markedly increased by extracts containing a particular phospholipid composition. However, this stimulation depends mainly upon the phospholipid composition of the fraction used as fermentation substrate. Fractions enriched mainly in phosphatidylinositol (Epikuron 110), at the expense of phosphatidylcholine, were the most efficient sources for phospholipid transfer protein production by A. oryzae. Maximal phospholipid transfer activity, as well as biomass production, were increased 4.1- and 9.7-fold, respectively, when cultures were supplemented with Epikuron 110 prepared from sunflower lecithin, as compared to glucose-control cultures.Key words: Aspergillus oryzae, industrial phospholipid sources, phospholipid transfer protein.

L'effet de sources de carbone industrielles sur la production de protéines de transfert de phospholipides a été testée. Les fractions phospholipidiques de différentes compositions, ont été préparées à partir de plusieurs plantes oléagineuses (soja, colza et tournesol) selon le procédé d'extraction et de purification développé par Lucas Meyer. Nous avons étudié l'effet de ces fractions sur l'activité de transfert de phospholipides présente dans les extraits cellulaires d'Aspergillus oryzae cultivé sur un milieu contenant ces fractions comme seule source de carbone. Dans ces conditions, l'activité de transfert de phospholipides est fortement augmentée. Cependant, cette augmentation est étroitement liée à la composition en phospholipides des fractions utilisées comme substrat de fermentation. Les fractions principalement enrichies en phosphatidylinositol (Epikuron 110), aux dépends de la phosphatidylcholine, sont les plus efficaces. L'activité de transfert de phospholipides ainsi que la biomasse, sont respectivement augmentées d'un facteur 4.1 et 9.7 lorsque les cultures sont supplémentées avec de l'Epikuron 110 de tournesol, en comparaison avec des cultures contrôles réalisées en présence de glucose.Mots clés : Aspergillus oryzae, source industrielle de phospholipides, protéine de transfert de phospholipides.

Keywords: Aspergillus oryzae; industrial phospholipid sources; phospholipid transfer protein; protéine de transfert de phospholipides; source industrielle de phospholipides

Document Type: Research Article

Publication date: 2001-07-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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