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Molecular strain typing of Campylobacter jejuni by pulsed-field gel electrophoresis in a single day

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Rapid molecular strain typing is critical for effective outbreak investigation and implementation of infection control measures. Pulsed-field gel electrophoresis is a highly discriminatory technique for Campylobacter jejuni, but generally requires 3–5 days. We describe a simplified protocol for pulsed-field gel electrophoresis that provides high quality typing of C. jejuni isolates in a single day.Key words: pulsed-field gel electrophoresis, Campylobacter jejuni, molecular epidemiology.

Le typage moléculaire rapide des souches est essentiel pour l'investigation efficace des épidémies et l'implantation de mesures appropriées de contrôle des infections. L'électrophorèse en champs pulsés est une technique hautement discriminante pour le Campylobacter jejuni, mais nécessite généralement 3–5 jours. Notre protocole simplifié d'électrophorèse en champs pulsés pour le C. jejuni permet d'obtenir des résultats de génotypage de haute qualité en une seule journée.Mots clés : électrophorèse en champs pulsés, Campylobacter jejuni, épidémiologie moléculaire.

Keywords: Campylobacter jejuni; molecular epidemiology; pulsed-field gel electrophoresis; électrophorèse en champs pulsés; épidémiologie moléculaire

Document Type: Research Article

Publication date: 2001-07-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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