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Rapid detection of Brevibacillus formosus BN53-1 in chicken feces

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Abstract:

Here we describe a rapid method for detecting the hydrogen sulfide-decomposing bacterium Brevibacillus formosus BN53–1 in chicken feces. The method, which can be adapted to the specific detection of a variety of useful eubacteria, is based on blot hybridization and polymerase chain reaction (PCR), and makes use of the genus or species hypervariable region of eubacterial 16S rDNA. The approximate limit of detection under the conditions we tested was 1.0 × 103 cells in 10 mg of chicken feces.Key words: Brevibacillus, detection methods, PCR, blot hybridization, 16S rDNA.

Nous décrivons ici une méthode rapide pour la détection dans les excréments de poulet de Brevibacillus formosus BN53-1, une bactérie décomposant le sulfure d'hydrogène. La méthode, qui peut être adaptée à la détection spécifique d'une variété d'eubactéries utiles, est basée sur l'hybridation de buvardages et sur le PCR, et tire parti de la région hypervariable de l'ADNr 16S des espèces et genres eubactériens. La limite approximative de détection dans les conditions que nous avons testées était de 1,0×103 cellules dans 10 mg d'excréments de poulet.Mots clés : Brevibacillus, méthodes de détection, PCR, hybridation de buvardages, ADNr 16S.[Traduit par la Rédaction]

Keywords: 16S rDNA; ADNr 16S; Brevibacillus; PCR; blot hybridization; detection methods; hybridation de buvardages; méthodes de détection

Document Type: Research Article

Publication date: May 1, 2001

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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