Possible involvement of Pseudomonas fluorescens and Bacillaceae in structural modifications of Tuber borchii fruit bodies

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Abstract:

Previous studies on Tuber borchii fruit bodies in early maturation stages suggested a role of bacteria in sporocarp structural modifications. In order to verify this hypothesis, in the present study we investigated by means of microbial and ultrastructural approaches, the bacterial population of T. borchii sporocarps from intermediate maturation phases to advanced decomposition stages, paying particular attention to chitinolytic and cellulolytic bacteria and to their relationships with ascii and ascospores. We found that Pseudomonas fluorescens and spore-forming Bacillaceae, both able to degrade cellulose and chitin, are present inside the sporocarps in all maturation stages investigated. Moreover, rod-shaped bacteria seem able to erode ascus walls and colonize the interior of ascii containing mature spores. These results suggest a possible role of these bacteria in the process of ascus opening. Moreover, the presence of P. fluorescens and Bacillaceae on isolated mature spores after decontamination suggests an intimate association between these bacteria and the ascospores.Key words: bacteria, cellulose, chitin, ectomycorrhiza.

Des études antérieures portant sur les corps de fructification de Tuber borchii dans leurs stades de mûrissement précoces ont attribué à des bactéries un rôle dans la modification structurelle des sporocarpes. Afin de vérifier cette hypothèse, nous avons examiné dans l'étude présente la population bactérienne des sporocarpes de T. borchii des phases de mûrissement intermédiaires aux stades de décomposition avancées, en empruntant des approches microbiennes et utrastructurelles. Nous avons porté une attention particulière aux bactéries chitinolytiques et cellulolytiques et à leurs relations avec les asques et les ascospores. Nous avons découvert que Pseudomonas fluorescens et les Bacilliaceae formant des spores, tous deux capables de dégrader la cellulose et la chitine, sont présents à l'intérieur des sporocarpes dans tous les stades de mûrissement examinés. En outre, des bactéries en forme de bâtonnets semblent être capables d'éroder les parois et de coloniser l'intérieur d'asques contenant des spores mûres. Ces résultats attribuent à ces bactéries un rôle potentiel dans le processus d'ouverture des asques. De plus, la présence de P. fluorescens et de Bacilliaceae sur des spores mûres isolées, après décontamination, évoquent une association intime entre ces bactéries et les ascospores.Mots clés: bactéries, cellulose, chitine, ectomycorhize.[Traduit par la Rédaction]

Keywords: bacteria; cellulose; chitin; ectomycorrhiza

Document Type: Research Article

Publication date: March 1, 2001

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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