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Selective isolation and distribution of the genus Planomonospora in soils

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Abstract:

For the screening of bioactive compounds and study of global distribution, a selective isolation method for Planomonospora strains by centrifugation from soil is examined. Planomonospora strains produced characteristic sporangia on the humic acid-trace salts gellan gum medium (pH 9.0) so that this genus was readily recognized on the isolation plate. High yields of motile spores were obtained by using a flooding solution containing 0.1% skim milk in 5 mM N-cyclohexyl-2-amino-ethanesulfonic acid (pH 9.0) followed by incubating the preparation at 32°C for 90 min, centrifuging it at 1000 × g for 10 min, and further incubation at 32°C for 60 min after centrifugation. By combining the techniques described above, we isolated 246 Planomonospora strains from 137 of the 1200 soil samples examined. Ninety-four percent of these strains were recovered from neutral to slightly alkaline soils (pH 7.0 to 9.0). Strains of P. venezuelensis group were obtained from 13 soil samples (1.1%), which were collected in Bolivia, Cyprus, Egypt, Greece, India, Japan, New Caledonia, and Turkey. Strains of this group appear widely distributed in the soil of tropical to temperate regions. To our knowledge, this is the first record that strains of this group have been isolated from a location other than Venezuela.Key words: Planomonospora, gellan gum, selective isolation, distribution, actinomycete.

Nous avons analysé une méthode d'isolation sélective à partir de la terre de souches de Planomonospora par centrifugation, dans le but de cribler ses composés bioactifs et d'étudier sa distribution globale. Les souches de Planomonospora ont produit des sporanges caractéristiques sur le milieu humique acide-traces salines et gomme gélifiante (pH 9,0), ce qui permit une identification facile sur la plaque d'isolation. Des hauts rendements de spores mobiles furent obtenus en utilisant une solution d'immersion renfermant 0,1% de lait écrémé dans 5 mM d'acide N-cyclohexyl-2-amino-ethanesulfonique (pH 9,0) suivi d'une incubation de la préparation à 32°C pendant 90 min, une centrifugation de 10 min à 1000 × g, puis une incubation supplémentaire à 32°C pendant 60 min. En combinant les techniques énoncées ci-haut, nous avons isolé 246 souches de Planomonospora à partir de 137 des 1200 échantillons de sol analysés. Quatre-vingt-quatorze pour-cents de ces souches ont été recueillies à partir de sols légèrement alcalins (pH 7,0 à 9,0). Les souches du groupe P. venezuelensis furent obtenues à partir de 13 échantillons de sol (1,1%). On les retrouva en Bolivie, Chypre, Égypte, Grèce, Inde, Japon, Nouvelle Calédonie et Turquie. Les souches appartenant à ce groupe semblent être généralement distribuées dans des régions au climat tropical à tempéré. À notre connaissance, ceci est la première mention de souches de ce groupe qui auraient été isolées ailleurs qu'au Vénézuela.Mots clés : Planomonospora, gomme gélifiante, isolation sélective, distribution, actinomyètes.[Traduit par la Rédaction]

Keywords: Planomonospora; actinomycete; actinomyètes; distribution; gellan gum; gomme gélifiante; isolation sélective; selective isolation

Document Type: Research Article

Publication date: March 1, 2001

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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