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Variability and interactions between endophytic bacteria and fungi isolated from leaf tissues of citrus rootstocks

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Fungi and bacteria were isolated from surface disinfected leaf tissues of several citrus rootstocks. The principal bacterial species isolated were Alcaligenes sp., Bacillus spp. (including B. cereus, B. lentus, B. megaterium, B. pumilus, and B. subtilis), Burkholderia cepacia, Curtobacterium flaccumfaciens, Enterobacter cloacae, Methylobacterium extorquens, and Pantoea agglomerans, with P. agglomerans and B. pumilus being the most frequently isolated species. The most abundant fungal species were Colletotrichum gloeosporioides, Guignardia citricarpa, and Cladosporium sp. Genetic variability between 36 endophytic bacterial isolates was analysed by the random amplified polymorphic DNA (RAPD) technique, which indicated that B. pumilus isolates were more diverse than P. agglomerans isolates, although genetic diversity was not related to the host plants. In vitro interaction studies between G. citricarpa isolates and the most frequently isolated endophytic bacteria showed that metabolites secreted by G. citricarpa have an inhibitory growth effect on some Bacillus species, and a stimulatory growth effect on P. agglomerans.Key words: endophytes, citrus, fungal-bacterial interaction, RAPD, diversity, Pantoea agglomerans, Bacillus pumilus, Guignardia citricarpa.

Des champignons et des bactéries ont été isolés de tissus de feuilles désinfectées en surface de plusieurs rhizomes de citron. Les principales espèces de bactérie isolées étaient Alcaligenes sp., Bacillus spp. (incluant B. cereus, B. lentus, B. megaterium, B. pumilus et B. subtilis), Burkholderia cepacia, Curtobacterium flaccumfaciens, Enterobacter cloacae, Methylobacterium extorquens et Pantoea agglomerans, P. agglomerans et B. pumilus furent les espèces retrouvées le plus souvent. Les espèces de champignons les plus courantes étaient Colletotrichum gloeosporioides, Guignardia citricarpa et Cladosporium sp. La variabilité génétique entre 36 isolats de bactéries endophytiques a été analysée par la technique du RAPD (ADN polymorphe amplifié au hasard), qui a indiqué que les isolats de B. pumilus étaient plus diversifiés que ceux de P. agglomerans, bien que la diversité génétique n'était pas liée aux plantes hôtes. Les études d'interaction in vitro entre les isolats de G. citricarpa et les bactéries endophytiques les plus souvent isolées a démontré que des métabolites sécrétés par G. citricarpa ont une activité inhibant la croissance chez certaines espèces du genre Bacillus, et ont une activité simulatrice pour la croissance de P. agglomerans.Mots clés : endophytes, citron, interaction bactérie-champignon, RAPD, diversité, Pantoea agglomerans, Bacillus pumilus, Guignardia citricarpa.[Traduit par la Rédaction]

Keywords: Bacillus pumilus; Guignardia citricarpa; Pantoea agglomerans; RAPD; citrus; diversity; endophytes; fungal-bacterial interaction

Document Type: Research Article

Publication date: March 1, 2001

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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