Functional integrity and structural stability of freeze-dried ectomycorrhizal fungi established through viability assays

Authors: Sundari S.K.; Adholeya A.

Source: Canadian Journal of Microbiology, Volume 47, Number 2, February 2001 , pp. 172-177(6)

Publisher: NRC Research Press

Abstract:

Lyophilized vegetative mycelium of ectomycorrhizal fungi was subjected to various viability tests to confirm functional integrity. Physical integrity of freeze-dried cultures was comparable to that of non-lyophilized cultures. Inter- and intraspecific variations in morphology, physiology, and metabolic rate were maintained after lyophilization. Maintenance of total protein content confirmed metabolic stability. According to the assays of viability, a plating assay and determination of total biomass confirmed stable mitotic activity of the freeze-dried cultures.Key words: lyophilization, physical integrity, structural stability, total protein, total biomass.

Le mycélium végétatif lyophilisé de champignons ectomycorrhizaux fut soumis à une batterie de tests afin d'attester de son intégrité fonctionnelle. L'intégrité physique de cultures lyophilisées était comparable à celle de cultures non lyophilisées. Les variations inter- et intraspécifiques dans la morphologie, la physiologie et le taux métabolique furent conservées après la lyophilisation. Le maintien du contenu total en protéines a permis de confirmer la stabilité métabolique. Selon les analyses de viabilité, un test d'ensemencement et une caractérisation de la biomasse ont confirmé la stabilité de l'activité miotique de cultures ayant été lyophilisées.Mots clés : lyophilisation, intégrité physique, stabilité structurelle, protéines totales, biomasse totale.[Traduit par la Rédaction]

Keywords: lyophilization; physical integrity; structural stability; total protein; total biomass; lyophilisation; intégrité physique; stabilité structurelle; protéines totales; biomasse totale

Language: English

Document Type: Research article

Publication date: 2001-02-01

• Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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