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Genetic diversity of Bradyrhizobium strains isolated from Arachis hypogaea

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Abstract:

Rhizobia are used exclusively in agricultural systems for enhancing the ability of legumes to fix atmospheric nitrogen. Knowledge about the indigenous population is necessary for the selection and application of inoculant strains. In this study, we have assessed the genetic diversity of Bradyrhizobium strains isolated from the host plant, Arachis hypogaea along the coastline of Tamil Nadu. Different populations collected from varying environmental conditions were analysed for salt and pH tolerance. Genetic diversity among the strains was studied using RAPD markers and PCR-RFLP of 16S rDNA and nifD genes. The approaches used in this study yielded consistent results, which revealed a high degree of heterogeneity among strains and detection of two distinct genetic groups.Key words: rhizobia, diversity, salinity, RAPD, PCR-RFLP.

Les rhizobiums sont utilisés exclusivement dans les systèmes agricoles pour améliorer la capacité des légumineuses à fixer l'azote atmosphérique. Une connaissance des populations indigènes est requise pour la sélection et l'application de souches servant d'inoculum. Dans cette étude, nous avons évalué la diversité génétique de souches de Bradyrhizobium isolées à partir de la plantes hôte, Arachis hypogaea, le long du littoral de Tamil Nadu. Les populations diverses recueillies dans des conditions environnementales variables ont été analysées pour leur tolérance aux sel et au pH. La diversité génétique parmi les souches fut étudiée en utilisant des marqueurs de RAPD et le PCR-RFLP de l'ADNr 16S et des gènes nifD. Les approches empruntées dans cette étude ont fourni des résultats fiables, qui révélèrent un haut degré d'hétérogénéité parmi les souches et qui ont permis la détection de deux groupes génétiques distincts.Mots clés : rhizobiums, diversité, salinité, RAPD, PCR-RFLP.[Traduit par la Rédaction]

Keywords: PCR-RFLP; RAPD; diversity; diversité; rhizobia; rhizobiums; salinity; salinité

Document Type: Research Article

Publication date: February 1, 2001

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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