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Determination of 1-aminocycopropane-1-carboxylic acid (ACC) to assess the effects of ACC deaminase-containing bacteria on roots of canola seedlings

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Previously, it was proposed that plant growth-promoting bacteria that possess the enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, can reduce the amount of ethylene produced by a plant and thereby promote root elongation. To test this model, canola seeds were imbibed in the presence of the chemical ethylene inhibitor, 2-aminoethoxyvinyl glycine (AVG), various strains of plant growth-promoting bacteria, and a psychrophilic bacterium containing an ACC deaminase gene on a broad host range plasmid. The extent of root elongation and levels of ACC, the immediate precursor of ethylene, were measured in the canola seedling roots. A modification of the Waters AccQ*Tag Amino Acid Analysis Method(tm) was used to quantify ACC in the root extracts. It was found that, in the presence of the ethylene inhibitor, AVG, or any one of several ACC deaminase-containing strains of bacteria, the growth of canola seedling roots was enhanced and the ACC levels in these roots were lowered.

Il fut proposé précédemment que des bactéries stimulant la croissance des plantes, qui possèdent l'enzyme 1-aminocylclopropane-1-carboxylate (ACC) déaminase, peuvent réduire la quantité d'éthylène produite par la plante et donc favoriser l'élongation de ses racines. Afin d'éprouver ce modèle, des graines de canola furent traitées en présence d'une inhibiteur chimique de l'éthylène, 2-aminoéthoxyvinyl glycine (AVG), de diverses souches de bactéries stimulatrices de la croissance des plantes, et d'une bactérie psychrophilique contenant un gène de l'ACC déaminase sur un plasmide de gamme d'hôte étendue. L'ampleur de l'élongation des racines et des niveaux d'ACC, le précurseur immédiat de l'éthylène, furent mesurés dans les racines des pousses de canola. Nous avons utilisé une version modifiée du Waters AccQ*Tag Amino Acid Analysis Method(tm) afin de quantifier l'ACC dans les extraits de racines. Nous avons découvert que, en présence de l'inhibiteur d'éthylène, AVG, ou de n'importe laquelle des diverses souches contenant l'ACC déaminase, la croissance des racines des pousses de canola était accrue et que les niveaux d'ACC dans ces racines étaient réduits.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: January 1, 2001

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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