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Changes in enzymatic activities involved in glucose metabolism by acyl-CoAs in Trypanosoma cruzi

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Abstract:

This study describes the effect of some saturated and unsaturated free fatty acids and acyl-CoA thioesters on Trypanosoma cruzi glucose 6-phosphate dehydrogenase and hexokinase activities. Glucose 6-phosphate dehydrogenase was sensitive to the destabilizing effect provoked by free fatty acids, while hexokinase remained unaltered. Glucose 6-phosphate dehydrogenase inhibition by free fatty acids was dependent on acid concentration and chain length. Both enzymes were inhibited when they were incubated with acyl-CoA thioesters. The acyl-CoA thioesters inhibited glucose 6-phosphate dehydrogenase at a lower concentration than the free fatty acids; the ligands glucose 6-phosphate and NADP+ afforded protection. The inhibition of hexokinase by acyl-CoAs was not reverted when the enzyme was incubated with ATP. The type of inhibition found with acyl-CoAs in relation to glucose 6-phosphate dehydrogenase and hexokinase suggests that this type inhibition may produce an in vivo modulation of these enzymatic activities.Key words: Trypanosoma cruzi, fatty acids, acyl-CoAs, glucose 6-phosphate dehydrogenase, hexokinase.

Dans cette étude, nous relatons l'effet de certains acides gras libres saturés et insaturés et de thioesters d'acyles-CoA sur les activités de la glucose 6-phosphate déshydrogénase et de l'hexokinase de Trypanosoma cruzi. La glucose 6-phosphate déshydrogénase était sensible aux effets déstabilisants causés par les acides gras libres, alors que l'hexokinase resta inchangée. L'inhibition de la glucose 6-phosphate déshydrogénase par les acides gras libres était dépendante de la concentration de l'acide et de la longueur de sa chaîne. Les deux enzymes ont été inhibées lorsque incubées avec des thioesters d'acyles-CoA. Les thioesters d'acyles-CoA ont inhibé la glucose 6-phosphate déshydrogénase à plus basses concentrations que les acides gras libres; les ligands de la 6-phosphate déshydrogénase et le NADP+ ont fourni une protection. L'inhibition de l'hexokinase par des acyles-CoA n'a pas été renversée lorsque l'enzyme fut incubée avec de l'ATP. Le type d'inhibition retrouvé chez l'acyle-CoA en lien avec la glucose 6-phosphate déshydrogénase et l'hexokinase suggère qu'elle pourrait induire une modulation in vivo de ces activités enzymatiques.Mots clés : Trypanosoma cruzi, acides gras, acyles-CoA, glucose 6-phosphate déshydrogénase, hexokinase.[Traduit par la Rédaction]

Keywords: Trypanosoma cruzi; acides gras; acyl-CoAs; acyles-CoA; fatty acids; glucose 6-phosphate dehydrogenase; glucose 6-phosphate déshydrogénase; hexokinase

Document Type: Research Article

Publication date: January 1, 2001

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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