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In vitro regeneration of Pinus pinaster adult trees

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Regeneration of adult conifer trees by means of in vitro culture has been the subject of intense study during the last 20 years. Propagation by tissue culture may become the best method for obtaining multiple identical trees and for capturing the genetic gain in breeding programs. However, the method has several problems related to phase change of trees (juvenile–adult) that limit its practical applications. In this study, shoot buds were collected from 20 maritime pine (Pinus pinaster Ait.) adult trees (>20years old) from November to March. Buds were cut transversely into 0.5–1.0cm slices and cultured on several media (DCR, WP and modified LP) supplemented with cytokinins (6-benzyladenine, metatopolin, or zeatin) at two concentrations (25 or 50mol/L). The highest organogenic response (axillary shoots formation ability) occurred on DCR medium supplemented with 25mol/L zeatin and metatopolin, and 25 or 50mol/L 6-benzyladenine. All shoots that regenerated on DCR medium with 25mol/L 6-benzyladenine developed healthy and well rooted plantlets. The ability to micropropagate adult trees represents a significant progress in the application of biotechnology to forest tree improvement programs, and it opens the possibility of using select trees in agroforestry areas under biotic or abiotic stress.

La régénération des conifères adultes au moyen de la culture in vitro a été beaucoup étudiée durant les 20 dernières années. La propagation par la culture de tissus pourrait devenir la meilleure méthode pour obtenir une multitude d’arbres identiques et conserver le gain génétique dans les programmes d’amélioration. Cependant, la méthode comporte plusieurs problèmes reliés au changement de phase des arbres (juvénile–adulte) qui limitent ses applications pratiques. Dans cette étude, des bourgeons foliaires ont été collectés sur 20 individus adultes (>20 ans) de Pinus pinaster Ait. de novembre à mars. Les bourgeons ont été coupés transversalement en tranches de 0,5 à 1 cm et cultivés sur plusieurs milieux (DCR, WP et LP modifié) enrichis de cytokinines (6-benzyleadénine, métatopoline ou zéatine) à deux concentrations (25 ou 50 mol/L). La plus forte réaction organogène (capacité de former des pousses axillaires) a été observée sur le milieu DCR enrichi de 25 mol/L de zéatine et de méta-topoline et de 25 ou 50 mol/L de 6-benzyleadénine. Toutes les pousses qui se sont régénérées sur le milieu DCR avec 25 ou 50 mol/L de 6-benzyleadénine ont développé des plantules saines et bien enracinées. La capacité de micropropager des arbres adultes constitue un progrès significatif dans l’application de la biotechnologie dans les programmes d’amélioration des arbres forestiers et ouvre la possibilité d’utiliser des arbres sélectionnés dans les régions agroforestières qui subissent des stress biotiques ou abiotiques.

Document Type: Research Article

Publication date: 2008-10-01

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  • Published since 1971, this monthly journal features articles, reviews, notes and commentaries on all aspects of forest science, including biometrics and mensuration, conservation, disturbance, ecology, economics, entomology, fire, genetics, management, operations, pathology, physiology, policy, remote sensing, social science, soil, silviculture, wildlife and wood science, contributed by internationally respected scientists. It also publishes special issues dedicated to a topic of current interest.
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