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Somatic embryogenesis in Greek fir

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Abstract:

To induce somatic embryogenesis of Greek fir, Abies cephalonica Loud., the influence of different cultivation media, sampling dates, and cold treatment storage were examined. The initiation frequencies ranged from 1% to 25%. The sampling date had a significant effect on the initiation of embryogenic cultures and a high frequency was observed within a 5week window. The effect of cone storage, composition of initiation media, and the medium by storage interaction also remained nonsignificant after 3 and 10months of proliferation growth. At the maturation phase, the highest tested concentrations of abscisic acid (ABA), 16 and 32mol/L, increased both the capability of embryogenic cell masses (ECMs) to produce embryos and the mean number of embryos. Of the two tested carbohydrates, maltose-containing media produced more embryos altogether (1943, on the average of 22.3 embryos per gram fresh mass) and 76% of the ECMs were able to produce embryos when compared with the corresponding values (152, on the average 3.0 embryos per gram fresh mass) and 15.3%, respectively, on sucrose-containing medium. However, 93.5% of embryos on maltose-containing medium died during the germination phase, whereas the corresponding percentage on sucrose-containing medium was 78.2%.

L’influence de différents milieux de culture, dates d’échantillonnage et traitements d’entreposage au froid a été examinée dans le but de provoquer l’embryogenèse somatique chez Abies cephalonica Loud. La fréquence d’initiation a varié de 1 à 25%. La date d’échantillonnage a eu un effet important sur l’initiation des cultures embryogènes et une fréquence élevée a été observée à l’intérieur d’une fenêtre de cinq semaines. Les effets de l’entreposage des cônes, de la composition du milieu d’initiation et de l’interaction entre le milieu et l’entreposage sont également demeurés non significatifs après trois à 10mois de croissance soutenue en phase de prolifération. À la phase de maturation, les concentrations d’acide abscissique (ABA) les plus élevées qui ont été testées, 16 et 32mol/L, ont augmenté tant la capacité des amas de cellules embryogènes de produire des embryons que le nombre moyen d’embryons. Des deux hydrates de carbone testés, les milieux qui contenaient du maltose ont globalement produit plus d’embryons, 1943 (moyenne de 22,3 embryons/g de poids frais) et de plus 76% des amas de cellules embryogènes étaient capables de produire des embryons. Par comparaison, les valeurs correspondantes sur les milieux contenant du sucrose étaient de 152 (moyenne de 3,0 embryons/g de poids frais) et 15,3%. Cependant, 93,5% des embryons sur le milieu contenant du maltose sont morts durant la phase de germination tandis que le pourcentage correspondant sur le milieu contenant du sucrose était de 78,2%.

Document Type: Research Article

Publication date: April 1, 2008

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  • Published since 1971, this monthly journal features articles, reviews, notes and commentaries on all aspects of forest science, including biometrics and mensuration, conservation, disturbance, ecology, economics, entomology, fire, genetics, management, operations, pathology, physiology, policy, remote sensing, social science, soil, silviculture, wildlife and wood science, contributed by internationally respected scientists. It also publishes special issues dedicated to a topic of current interest.
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