Micropropagation with a novel pattern of adventitious rooting in Formosan sweetgum

Authors: Ďurkovič, Jaroslav; Pichler, Viliam; Lux, Alexander

Source: Canadian Journal of Forest Research, Volume 35, Number 12, December 2005 , pp. 2775-2780(6)

Publisher: NRC Research Press

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Abstract:

Cultures of the Formosan sweetgum (Liquidambar formosana Hance) were established from axillary buds of intact plants and from petiole segments of in vitro grown leaves. Petiole segments were generally slower than axillary buds to form multiple shoot cultures. Thidiazuron in combination with 6-benzylaminopurine produced on average up to 9.4 shoots per explant. Shoot elongation was significantly affected by the concentration of 6-benzylaminopurine, regardless of auxin addition to the medium. Adventitious rooting occurred up to 90%. An unexpected pattern of adventitious rhizogenesis was observed from the aerial parts of microshoots not in contact with the rooting medium, including leaf blades and petiole axils. In vitro adventitious root formation from the leaf blade was associated with parenchyma cells surrounding the main vein. The regenerated plants established ex vitro did not show any detectable morphological variation and no aerial roots were formed.

Des cultures de liquidambar de Formose (Liquidambar formosana Hance) ont été réalisées à partir des bourgeons axillaires de plantes intactes et à partir de segments de pétioles provenant de feuilles cultivées in vitro. Les segments de pétioles étaient généralement plus lents que les bourgeons axillaires pour former des cultures de pousses multiples. Le thidiazuron, combiné au 6-benzylaminopurine, a permis de produire jusqu'à 9,4 pousses par explant en moyenne. L'élongation des pousses était affectée de façon significative par la concentration de 6-benzylaminopurine, avec ou sans l'addition d'auxines au milieu de culture. Des racines adventives ont été produites dans 90 % des cas. Un patron inattendu de rhizogenèse adventive à partir des parties aériennes des micropousses non en contact avec le milieu d'enracinement a été observé incluant les limbes foliaires et l'axe des pétioles. La formation in vitro de racines adventives à partir du limbe foliaire était associée aux cellules de parenchyme entourant la nervure médiane. Les plants régénérés qui ont été établis ex vitro n'ont pas montré de variation morphologique détectable et aucune racine aérienne ne s'est formée.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: December 1, 2005

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