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Variation of paternal contribution in a seed orchard of Cryptomeria japonica determined using microsatellite markers

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Abstract:

We investigated gene flow and pollen contamination in a seed orchard of Cryptomeria japonica D. Don using six microsatellite markers. The quality of a seed crop is determined by many factors, including the rate of pollen contamination from outside sources, degree of self-fertilization, and equality of the clones as pollen donors. The surveyed seed orchard consisted of 62 clones and a total of 360 ramets. The seeds from 12 mother trees in the seed orchard were investigated using six highly polymorphic loci with high multipaternity exclusion probability (0.999). The estimated average pollen contamination rate was high at 47.78% (±4.12%), and the clonal self-fertilization rate was 2.22% (±0.94%) when we assumed that null alleles were present. The high pollen contamination rate could dramatically reduce the quality of the seed crop. The contamination rate differed from tree to tree, but the differences were not related to the location of each individual. The levels of contribution as pollen donors differed significantly amongst clones in the seed orchard. Differences in flowering phenology and relative pollen fecundity may be responsible for the differences in paternal contributions. The effective distance of pollen dispersal in this C. japonica seed orchard seemed to be greater than previously estimated.

Les auteurs ont évalué le flux génique et la contamination pollinique au sein d'un verger à graines de Cryptomeria japonica D. Don à l'aide de six marqueurs microsatellites. La qualité de la production semencière est déterminée par plusieurs facteurs dont le taux de contamination pollinique exogène, le degré d'autogamie et l'équilibre des contributions polliniques des différents clones. Le verger à graines étudié était constitué de 62 clones comprenant un total de 360 ramets. Les semences de 12 arbres maternels du verger à graines ont été étudiées à l'aide de six loci hautement polymorphes caractérisés par une forte probabilité (0,999) d'exclusion de paternité multiple. Avec une valeur de 47,78 % (±4,12 %), le taux moyen estimé de contamination pollinique était élevé. Le taux d'autogamie intra-clonal était de 2,22 % (±0,94 %) en assumant que les allèles nuls étaient présents. Le fort taux de contamination pollinique pourrait réduire de façon dramatique la qualité de la production semencière. Le taux de contamination variait d'un arbre à l'autre, mais les différences ne découlaient pas de l'emplacement de chaque individu. Les contributions polliniques variaient de façon significative parmi les clones du verger à graines. Des différences de phénologie florale et de fécondité pollinique relative pourraient expliquer les différences observées entre les contributions paternelles. La distance effective de dispersion du pollen au sein de ce verger à graines de C. japonica semblait être plus grande que les estimations antérieures.

Document Type: Research Article

Publication date: August 1, 2004

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  • Published since 1971, this monthly journal features articles, reviews, notes and commentaries on all aspects of forest science, including biometrics and mensuration, conservation, disturbance, ecology, economics, entomology, fire, genetics, management, operations, pathology, physiology, policy, remote sensing, social science, soil, silviculture, wildlife and wood science, contributed by internationally respected scientists. It also publishes special issues dedicated to a topic of current interest.
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