Skip to main content

Cryopreservation of Pinus radiata zygotic embryo cotyledons: effect of storage duration on adventitious shoot formation and plant growth after 2 years in the field

Buy Article:

$50.00 plus tax (Refund Policy)

Abstract:

This work is the first report of the cryopreservation of conifer cotyledons without cryoprotectants and their subsequent shoot regeneration and successful establishment of a field trial. Multiple genotypes of radiata pine (Pinus radiata D. Don) embryo cotyledons were stored in liquid nitrogen following a desiccation treatment. Cotyledons that had been stored in liquid nitrogen for 7, 14, and 28 days were compared with noncryopreserved cotyledons for adventitious shoot production, root formation on the shoots, and plant growth after 2 years in the field. Of the 72 genotypes tested, 79%–87% of them produced shoots on at least one treatment and 59% of them produced shoots on all treatments. Rooting rates of shoots were not affected by treatment, with the cryopreserved treatments rooting as well as the noncryopreserved controls. Height growth of plants in the nursery was similar across all treatments after 2 years but was influenced by setting date. The higher genotype capture possible with adventitious methodologies, compared with that of somatic embryogenesis, and the preservation of juvenile characteristics while material is stored in liquid nitrogen make adventitious methods worthy of intensive study for possible commercial application.

Cet article est le premier à rapporter la cryoconservation de cotylédons de conifères sans l'utilisation de cryoprotecteurs, la régénération subséquente de pousses et la réussite de leur établissement dans un test au champ. Plusieurs génotypes d'embryons de cotylédons de Pinus radiata D. Don ont été entreposés dans l'azote liquide après un traitement de dessiccation. Les cotylédons qui avaient été entreposés dans l'azote liquide pendant 7, 14 et 28 jours ont été comparés à des cotylédons non cryoconservés pour la production de pousses adventives, la formation de racines sur les pousses et la croissance des plants après deux ans au champ. Des 72 génotypes testés, 79–87 % de ceux-ci ont produit des pousses dans au moins un traitement et 59 % dans tous les traitements. Le taux d'enracinement des pousses n'était pas affecté par les traitements; l'enracinement était le même que les tissus aient été cryoconservés ou non. La croissance en hauteur des plants en pépinière était similaire dans tous les traitements après deux ans mais était influencée par la date de mise en terre. La conservation de plus de génotypes qui est rendue possible grâce aux méthodes utilisant des tissus adventifs, comparativement à l'embryogenèse somatique, et la préservation des caractères juvéniles pendant que le matériel est conservé dans l'azote liquide justifient l'étude plus poussée de ces méthodes qui pourraient avoir une application commerciale.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: March 1, 2004

More about this publication?
  • Published since 1971, this monthly journal features articles, reviews, notes and commentaries on all aspects of forest science, including biometrics and mensuration, conservation, disturbance, ecology, economics, entomology, fire, genetics, management, operations, pathology, physiology, policy, remote sensing, social science, soil, silviculture, wildlife and wood science, contributed by internationally respected scientists. It also publishes special issues dedicated to a topic of current interest.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites
nrc/cjfr/2004/00000034/00000003/art00009
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more