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No evidence for large differences in genomic methylation between wild and hatchery steelhead (Oncorhynchus mykiss)

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Abstract:

When salmonid fish that have been raised in hatcheries spawn in the wild, they often produce fewer surviving adult offspring than wild fish. Recent data from steelhead (Oncorhynchus mykiss) in the Hood River (Oregon, USA) show that even one or two generations of hatchery culture can result in dramatic declines in fitness. Although intense domestication selection could cause such declines, it is worth considering alternative explanations. One possibility is heritable epigenetic changes induced by the hatchery environment. Here, we show, using methylation-sensitive amplified fragment length polymorphism, that hatchery and wild adult steelhead from the Hood River do not appear to differ substantially in overall levels of genomic methylation. Thus, although altered methylation of specific DNA sites or other epigenetic processes could still be important, the hatchery environment does not appear to cause a global hypo- or hypermethylation of the genome or create a large number of sites that are differentially methylated.

Lorsque des poissons salmonidés élevés en pisciculture se reproduisent en nature, ils produisent souvent moins de rejetons survivants que les poissons sauvages. Des données récentes sur la truite arc-en-ciel anadrome (Oncorhynchus mykiss) dans la rivière Hood (Oregon, É.-U.) montrent que même une ou deux générations en pisciculture peuvent entraîner des réductions spectaculaires de fitness. Bien qu’une sélection intense reliée à la domestication puisse causer de tels déclins, il est important d’examiner les hypothèses de rechange. Parmi les possibilités, il y a les changements épigénétiques héritables causés par l’environnement de pisciculture. Nous montrons ici, à l’aide du polymorphisme de la longueur des fragments amplifiés sensible à la méthylation, que les truites arc-en-ciel anadromes de pisciculture et les truites sauvages de la Hood ne semblent pas différer de manière importante dans leurs niveaux globaux de méthylation génomique. Ainsi, alors que la méthylation altérée de certains sites d’ADN ou d’autres processus épigénétiques pourraient quand même être importants, le milieu de pisciculture ne semble causer ni une hypo-, ni une hyper-méthylation globale du génome, ni créer un nombre élevé de sites à méthylation distincte.

Document Type: Research Article

Publication date: 2010-02-01

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  • Published continuously since 1901 (under various titles), this monthly journal is the primary publishing vehicle for the multidisciplinary field of aquatic sciences. It publishes perspectives (syntheses, critiques, and re-evaluations), discussions (comments and replies), articles, and rapid communications, relating to current research on cells, organisms, populations, ecosystems, or processes that affect aquatic systems. The journal seeks to amplify, modify, question, or redirect accumulated knowledge in the field of fisheries and aquatic science. Occasional supplements are dedicated to single topics or to proceedings of international symposia.
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