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Bacteria associated with the mucus layer of Merlangius merlangus (whiting) as biological tags to determine harvest location

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Abstract:

We describe a new technique to determine the harvest location of Merlangius merlangus (whiting) based on exploiting the natural bacterial populations associated with the mucus layer of fish and surrounding seawater as biological tags. Bacterial community profiles from the outer and mouth mucus and the surrounding seawater were characterized by terminal restriction fragment length polymorphism (T-RFLP), and fish harvest location was predicted based on T-RFLP profile comparisons between fish and water and between individual fish. Fish harvest location was not resolved reliably based on analysis of the shared seawater and outer-mucus markers. However, comparisons based on shared seawater and mouth-mucus markers placed fish within 1.92 (± standard error (SE) of 0.31) stations (p< 0.002), equivalent to 153.0km, of their known harvest location. Fish placement resolution was further increased when fish-to-fish bacterial assemblage comparisons were made. Based on outer-mucus, mouth-mucus, and combined outer- and mouth-mucus analysis, fish were placed within 1.00 (±SE 0.27), 1.55 (±SE 0.28), and 0.71 (±SE 0.24) (p< 0.04) stations, equivalent to 79.7, 123.5, and 57.3km, respectively, of their known harvest location.

Nous décrivons une nouvelle technique pour déterminer le lieu de récolte de Merlangius merlangus (merlus) basée sur l’utilisation des peuplements naturels de bactéries associés à la couche de mucus des poissons et à l’eau de mer environnante comme étiquettes biologiques. Nous avons caractérisé les profils des communautés bactériennes du mucus externe et buccal ainsi que de l’eau de mer environnante à l’aide du polymorphisme de la longueur des fragments de restriction terminaux (T-RFLP); nous avons ensuite prédit le lieu de capture d’après la comparaison des profils T-RFLP entre les poissons et l’eau et entre les poissons individuels. Il n’est pas possible de déterminer de manière fiable le lieu de récolte des poissons à partir de l’analyse des marqueurs communs de l’eau de mer et du mucus externe. Cependant, les comparaisons basées sur les marqueurs communs de l’eau de mer et du mucus oral permettent de placer les poissons à moins de 1,92 (± écart type (ET) de 0,31) station (p < 0,0002), soit l’équivalent de 153,0 km, de leur point connu de récolte. Une comparaison des communautés bactériennes entre les poissons accroît davantage la résolution de l’identification des points de capture. Selon que l’analyse est basée sur le mucus externe, le mucus oral, ou une combinaison de mucus externe et oral, les poissons sont assignés à respectivement moins de 1,00 (±ET 0,27), 1,55 (±ET 0,28) et 0,71 (±ET 0,24) (p < 0,04) stations, soit l’équivalent de 79,7, 123,5 et 57,3 km, de leur point connu de récolte.

Document Type: Research Article

Publication date: May 1, 2009

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  • Published continuously since 1901 (under various titles), this monthly journal is the primary publishing vehicle for the multidisciplinary field of aquatic sciences. It publishes perspectives (syntheses, critiques, and re-evaluations), discussions (comments and replies), articles, and rapid communications, relating to current research on cells, organisms, populations, ecosystems, or processes that affect aquatic systems. The journal seeks to amplify, modify, question, or redirect accumulated knowledge in the field of fisheries and aquatic science. Occasional supplements are dedicated to single topics or to proceedings of international symposia.
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