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Comparison of three microquantity techniques for measuring total lipids in fish

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To measure lipids in juvenile and adult fishes, we refined three microquantity approaches (microgravimetric, microcolorimetric sulfophosphovanillan (SPV), and Iatroscan thin layer chromatography– flame ionization detection (TLC–FID)) that were originally developed to measure lipids in small aquatic invertebrates. We also evaluated their precision and comparability by quantifying the total lipid content of age-1+ walleye (Sander vitreus), yellow perch (Perca flavescens), and lake whitefish (Coregonus clupeaformis) collected in Lake Erie (US–Canada), Lake Michigan (USA), and Muskegon Lake (Michigan, USA). Our findings demonstrate that (i) microquantity approaches provide estimates of total lipids in juvenile and adult fishes similar to those of more traditional macroquantity approaches, (ii) the microcolorimetric SPV and microgravimetric approaches produce near identical estimates of total lipid content, and (iii) the Iatroscan TLC–FID approach underestimates total lipids relative to the other approaches for individuals with high lipid levels. Ultimately, our research makes available additional techniques for measuring total lipid content of fishes that are less expensive than traditional techniques, owing to a reduced need for large quantities of samples and solvents.

Afin de doser les lipides dans les poissons juvéniles et adultes, nous raffinons trois méthodologies microquantitatives (microgravimétrie, microcolorimétrie à sulfophosphovanilline (SPV) et chromatographie sur couche mince Iatroscan couplée à un détecteur à ionisation de flamme (TLC–FID)) mises au point au départ pour mesurer les lipides dans de petits invertébrés aquatiques. Nous avons évalué leur précision et les avons comparées entre elles en dosant le contenu en lipides totaux de dorés (Sander vitreus), de perchaudes (Perca flavescens) et de grands corégones (Coregonus clupeaformis) d'âge 1+ récoltés aux lacs Érié (E.-U. – Canada), Michigan (É.-U.) et Muskegon (Michigan, É.-U.). Nos observations démontrent que (i) les méthodes microquantitatives fournissent des estimations des lipides totaux chez les poissons juvéniles et adultes semblables à celles des méthodologies macroquantitatives plus courantes, (ii) les méthodologies microcolorimétriques SPV et microgravimétriques donnent des estimations presque identiques du contenu lipidique total et (iii) la méthodologie TLC–FID Iatroscan sous-estime les lipides totaux par comparaison aux autres méthodes chez les individus qui ont un fort contenu lipidique. En fin de compte, notre recherche rend disponibles de nouvelles techniques pour mesurer le contenu lipidique total chez les poissons, qui sont moins coûteuses que les techniques habituelles, parce qu’elles requièrent de moins grandes quantités d'échantillons et de solvants.

Document Type: Research Article

Publication date: October 1, 2008

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  • Published continuously since 1901 (under various titles), this monthly journal is the primary publishing vehicle for the multidisciplinary field of aquatic sciences. It publishes perspectives (syntheses, critiques, and re-evaluations), discussions (comments and replies), articles, and rapid communications, relating to current research on cells, organisms, populations, ecosystems, or processes that affect aquatic systems. The journal seeks to amplify, modify, question, or redirect accumulated knowledge in the field of fisheries and aquatic science. Occasional supplements are dedicated to single topics or to proceedings of international symposia.
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