Ca2+ handling and oxidative capacity are greatly impaired in swimming muscles of hatchery-reared versus wild Atlantic salmon (Salmo salar)

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Abstract:

The swimming capacity of fish is strongly associated with muscle performance, although the prerequisites for effective movements have not been fully described at the molecular level. To compare the condition of swimming musculature of hatchery-reared Atlantic salmon (Salmo salar) with that of wild fish, we analyzed the relative level of two excitation–contraction coupling components (i.e., dihydropyridine receptor (DHPR) and ryanodine receptor (RyR)) and the oxidative capacity of muscles with histochemical and Western blot methods. The density of DHPR and RyR was considerably higher in swimming muscles of wild fingerlings (age 0+) (109.8% and 123.3% in red muscle; 128.6% and 186.0% in white muscle, respectively) and yearlings (age 1+) (153.5% and 459.1% in red muscle; 131.2% and 858.4% in white muscle) as compared with those in reared fish. Similar difference was also observed in the oxidative capacity of muscles. Moreover, the oxidative activity correlated positively with the level of DHPR and RyR. Our data indicate that calcium handling, as well as oxidative capacity of swimming muscles of reared salmon, is clearly separable from the corresponding capacities of wild fish. We suggest that the observed alteration is a major contributing factor to the well-documented differences in swimming ability between wild and hatchery-reared salmon.

La capacité de nage des poissons est fortement reliée à la performance musculaire, bien que les conditions préalables au niveau moléculaire de mouvements efficaces n’aient pas été complètement établies. Dans notre étude, afin de comparer la condition de la musculature de nage chez des saumons atlantiques (Salmo salar) élevés en pisciculture et des saumons sauvages, nous analysons les niveaux relatifs de deux composantes du couplage excitation–contraction, c’est-à-dire le récepteur de la dihydropyridine (DHPR) et le récepteur de la ryanodine (RyR), ainsi que la capacité oxydative des muscles à l’aide de méthodes histochimiques et de buvardage Western. Les densités du DHPR et du RyR sont considérablement plus élevées dans les muscles de la nage des alevins sauvages (age 0+) (respectivement 109,8 % et 123,3 % dans le muscle rouge et 128,6 % et 186,0 % dans le muscle blanc) et des saumons d’un an sauvages (age 1+) (respectivement 153,5 % et 459,1 % dans le muscle rouge et 131,2 % et 858,4 % dans le muscle blanc) par comparaison aux poissons de pisciculture. Il existe aussi des différences similaires dans la capacité oxydative des muscles. De plus, il y a une corrélation positive entre l’activité oxydative et les niveaux de DHPR et de RyR. En conclusion, nos données indiquent que la gestion du calcium, de même que la capacité oxydative des muscles de nage des poissons de pisciculture, sont nettement différentes des capacités correspondantes chez les poissons sauvages. Nous croyons que l’altération que nous observons est un des facteurs importants qui expliquent les différences bien connues de capacité de nage chez les poissons sauvages et les poissons de pisciculture.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: January 1, 2008

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  • Published continuously since 1901 (under various titles), this monthly journal is the primary publishing vehicle for the multidisciplinary field of aquatic sciences. It publishes perspectives (syntheses, critiques, and re-evaluations), discussions (comments and replies), articles, and rapid communications, relating to current research on cells, organisms, populations, ecosystems, or processes that affect aquatic systems. The journal seeks to amplify, modify, question, or redirect accumulated knowledge in the field of fisheries and aquatic science. Occasional supplements are dedicated to single topics or to proceedings of international symposia.
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