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The digestive and metabolic enzyme activity profiles of a nonmetamorphic marine fish species: effects of feed type and feeding level

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We investigated activity levels of metabolic and digestive enzymes in Atlantic wolffish (Anarhichas lupus) and their relationships with growth, ration level, and type of food during the first 50 days after hatch. Newly hatched wolffish were divided among three experimental groups differing in feed and ration (formulated feed in excess (FF), a maintenance ration of Artemia (LA), and Artemia in excess (EA)) that generated different growth rates. A principal component analysis revealed that activities of the glycolytic enzymes lactate dehydrogenase (LDH) and pyruvate kinase (PK) were associated with mass gain, while those of the aerobic enzymes citrate synthase and aspartate aminotransferase (AAT), and digestive enzymes (lipase and trypsin) were related to time (days) after hatch. Food restriction or food type allowed the observation of a direct relationship between the activities of trypsin and those of associated metabolic enzymes AAT and glutamate dehydrogenase in the LA group (Pearson's R of 0.71 and 0.59, respectively), as well as between the activities of amylase and those of LDH and PK (Pearson's R of 0.62 and 0.48, respectively) in the FF group. The adaptative importance of these patterns during early development of wolffish and their relationship to feeding conditions are examined.

Les effets de la croissance, du stade de développement, du type d'aliment et de la restriction alimentaire sur le niveau d'activité de certaines enzymes métaboliques et digestives ont été évalués chez le loup Atlantique (Anarhichas lupus) durant les 50 jours post-éclosion. Des taux de croissance différents ont été atteints avec succès chez des loups nouvellement éclos en utilisant une combinaison de différents types d'aliments et de taux d'alimentation: moulée commerciale en excès (FF), Artemia faible ration (LA) et Artemia en excès (EA). Une analyse en composantes principales a révélé que l'expression des enzymes glycolytiques (lactate déshydrogénase (LDH) et pyruvate kinase (PK)) est associée au gain en masse, alors que le développement des enzymes du métabolisme aérobie (citrate synthase et aspartate aminotransférase (AAT)) et des enzymes digestives (lipase et trypsine) est plus corrélé au temps depuis l'éclosion (exprimé en jours post-éclosion). La restriction alimentaire et le type d'aliment ont permis l'observation d'un lien direct entre l'activité de la trypsine et les deux enzymes associées, AAT et glutamate déshydrogénase (Pearson's R de 0,71 et 0,59, respectivement chez le groupe LA) et entre l'amylase, la LDH et la PK (Pearson's R de 0,62 et 0,48, respectivement). Les patrons de développement des enzymes en fonction des conditions nutritionnelles sont examinés en fonction de la stratégie adaptative.

Document Type: Research Article

Publication date: June 29, 2007

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  • Published continuously since 1901 (under various titles), this monthly journal is the primary publishing vehicle for the multidisciplinary field of aquatic sciences. It publishes perspectives (syntheses, critiques, and re-evaluations), discussions (comments and replies), articles, and rapid communications, relating to current research on cells, organisms, populations, ecosystems, or processes that affect aquatic systems. The journal seeks to amplify, modify, question, or redirect accumulated knowledge in the field of fisheries and aquatic science. Occasional supplements are dedicated to single topics or to proceedings of international symposia.
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