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Genetic effects of ELISA-based segregation for control of bacterial kidney disease in Chinook salmon (Oncorhynchus tshawytscha)

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We evaluated genetic variation in ability of Chinook salmon (Oncorhynchus tshawytscha) to resist two bacterial pathogens: Renibacterium salmoninarum, the agent of bacterial kidney disease (BKD), and Listonella anguillarum, an agent of vibriosis. After measuring R. salmoninarum antigen in 499 adults by enzyme-linked immunosorbent assay (ELISA), we mated each of 12 males with high or low antigen levels to two females with low to moderate levels and exposed subsets of their progeny to each pathogen separately. We found no correlation between R. salmoninarum antigen level in parents and survival of their progeny following pathogen exposure. We estimated high heritability for resistance to R. salmoninarum (survival h2 = 0.890 ± 0.256 (mean ± standard error)) independent of parental antigen level, but low heritability for resistance to L. anguillarum (h2 = 0.128 ± 0.078). The genetic correlation between these survivals (rA = –0.204 ± 0.309) was near zero. The genetic and phenotypic correlations between survival and antigen levels among surviving progeny exposed to R. salmoninarum were both negative (rA = –0.716 ± 0.140; rP = –0.378 ± 0.041), indicating that variation in antigen level is linked to survival. These results suggest that selective culling of female broodstock with high antigen titers, which is effective in controlling BKD in salmon hatcheries, will not affect resistance of their progeny.

Nous évaluons la variation génétique de la capacité de saumons chinook (Oncorhynchus tshwawytscha) à résister à deux pathogènes bactériens, Renibacterium salmoninarum, l'agent de la maladie bactérienne du rein (BKD), et Listonella anguillarum, un agent de la vibriose. Après avoir mesuré l'antigène R. salmoninarum chez 499 adultes par dosage immuno-enzymatique ELISA, nous avons accouplé chacun de 12 mâles possédant des niveaux haut ou bas d'antigène avec deux femelles ayant des niveaux bas à modéré; nous avons ensuite exposé des sous-ensembles de leurs rejetons à chaque pathogène séparément. Il n'y a pas de corrélation entre le niveau d'antigèneR. salmoninarum des parents et la survie des rejetons après une exposition aux pathogènes. L'héritabilité de la résistance à R. salmoninarum (survie h2 = 0,890 ± 0,256 (moyenne ± erreur type)) est élevée, quel que soit le niveau d'antigène des parents, mais celle de la résistance à L. anguillarum (h2 = 0,128 ± 0,078) est faible. La corrélation génétique entre ces survies (rA = –0,204 ± 0,309) est presque nulle. Les corrélations génétique et phénotypique entre la survie et les niveaux d'antigène chez les rejetons qui survivent à l'exposition à R. salmoninarum sont toutes deux négatives (rA = –0,716 ± 0,140; rP = –0,378 ± 0,041), ce qui indique que la variation du niveau d'antigène est relié à la survie. Ces résultats laissent croire que la sélection de stocks reproducteurs femelles ayant un fort titre d'antigène, une mesure efficace pour contrôler BKD dans les élevages de saumons, n'affectera pas la résistance à la maladie chez leurs rejetons.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: 2006-12-01

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  • Published continuously since 1901 (under various titles), this monthly journal is the primary publishing vehicle for the multidisciplinary field of aquatic sciences. It publishes perspectives (syntheses, critiques, and re-evaluations), discussions (comments and replies), articles, and rapid communications, relating to current research on cells, organisms, populations, ecosystems, or processes that affect aquatic systems. The journal seeks to amplify, modify, question, or redirect accumulated knowledge in the field of fisheries and aquatic science. Occasional supplements are dedicated to single topics or to proceedings of international symposia.
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