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Characterization of phosphorus in sequential extracts from lake sediments using 31P nuclear magnetic resonance spectroscopy

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Abstract:

Phosphorus (P) compounds in three different lake surface sediments were extracted by sequential P extraction and identified by 31P nuclear magnetic resonance (31P NMR) spectroscopy. The extraction procedure primarily discriminates between inorganic P-binding sites but most extraction steps also contained P not reacting (nrP) with the molybdenum complex during P analyses. In all three lakes, the nrP dominated in the NaOH extracts. Nonreactive P from the dystrophic lake was dominated by potentially recalcitrant P groups such as orthophosphate monoesters, while the nrP in the two more productive lakes also contained polyphosphates, pyrophosphate, and organic P groups such as P lipids and DNA-P that may be important in remineralization and recycling to the water column. In addition, polyphosphates showed substantial dynamics in settling seston. The Humic-P pools (P associated with humic acids) showed strong signals of orthophosphate monoesters in all three lakes, which supported the assumption that P-containing humic compounds are indeed recovered in this fraction, although other organic P forms are also present. Thus, in addition to expanding the understanding of which organic P forms that are present in lake sediments, the 31P NMR technique also demonstrated that the chemical extraction procedure may provide some quantification of recalcitrant versus labile organic P forms.

Nous avons extrait les composés phosphorés (P) des sédiments lacustres superficiels de trois lacs différents par extraction séquentielle du P et les avons identifiés par spectroscopie de résonnance magnétique nucléaire 31P (31P NMR). Le processus d'extraction discrimine surtout entre les divers sites de liaison du P inorganique, mais la plupart des étapes de l'extraction contiennent aussi du P (nrP) qui ne réagit pas au complexe de molybdène durant l'analyse de P. Dans les trois lacs, le nrP domine dans les extraits au NaOH. Le P non réactif dans le lac dystrophe est dominé par les groupes P potentiellement récalcitrants, tels que les monoesters d'orthophosphate, alors que le nrP dans les deux lacs plus productifs contient aussi des polyphosphates, du pyrophosphate et des groupes organiques de P, tels que les P-lipides et l'ADN-P qui peuvent être d'importance pour la reminéralisation et le recyclage vers la colonne d'eau. Il y en plus une importante dynamique des polyphosphates dans le seston en sédimentation. On trouve dans les pools de P humique un fort signal de monoesters d'orthophosphate dans les trois lacs, ce qui appuie l'hypothèse selon laquelle les composés humiques qui contiennent du P sont en fait récupérés dans cette fraction, bien que d'autres formes organiques de P soient aussi présentes. Ainsi, non seulement la technique 31P NMR permet de mieux connaître les formes organiques de P présentes dans les sédiments lacustres, mais elle démontre que la procédure d'extraction chimique peut fournir un bilan quantitatif approximatif des formes organiques de P récalcitrantes par rapport aux formes labiles.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: August 1, 2006

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  • Published continuously since 1901 (under various titles), this monthly journal is the primary publishing vehicle for the multidisciplinary field of aquatic sciences. It publishes perspectives (syntheses, critiques, and re-evaluations), discussions (comments and replies), articles, and rapid communications, relating to current research on cells, organisms, populations, ecosystems, or processes that affect aquatic systems. The journal seeks to amplify, modify, question, or redirect accumulated knowledge in the field of fisheries and aquatic science. Occasional supplements are dedicated to single topics or to proceedings of international symposia.
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