A comparison of population structure in black rockfish (Sebastes melanops) as determined with otolith microchemistry and microsatellite DNA

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Abstract:

The black rockfish (Sebastes melanops) is a long-lived (≤50 years) viviparous species with an extended pelagic larval period (3–5 months) and a broad continental distribution. Prolonged larval periods have been assumed to lead to widespread dispersal resulting in minimal population structure. We tested this assumption by comparing otolith microchemistry and microsatellite DNA of adult black rockfish from four locations in Oregon and Washington. We observed significant differences among locations in element-to-Ca ratios (Mg:Ca, Mn:Ca, Zn:Ca, Sr:Ca, and Ba:Ca) at the otolith edge, which represented the last year of life. Discriminant function analysis, based on otolith element-to-Ca ratios classified, on average, 64% of the fish to collection location, suggesting that the majority of individuals from these locations did not mix. Using microsatellite DNA, we observed significant genetic differences among adults collected 340–460 km apart (FST = 0.018 ± 0.004). Using five and seven loci, 63% and 75% of the fish, respectively, were correctly assigned to collection location. These two techniques provided corroborative and complementary information on the population structure of S. melanops.

Le sébaste noir (Sebastes melanops) est une espèce vivipare à grande longévité (≤50 ans) qui possède une période de vie larvaire pélagique prolongée (3–5 mois) et une vaste répartition continentale. On assume généralement qu’une période larvaire prolongée entraîne une vaste répartition qui mène à une structure démographique minimale. Nous avons vérifié cette supposition en comparant la microchimie des otolithes et l’ADN microsatellite des sébastes noirs à quatre sites en Oregon et au Washington. Il y a des différences significatives d’un site à l’autre des rapports des éléments sur le calcium (Mg:Ca, Mn:Ca, Zn:Ca, Sr:Ca et Ba:Ca) en bordure des otolithes, zone qui correspond à la dernière année de vie. Une analyse des fonctions discriminantes basée sur les rapports éléments/calcium classe en moyenne 64 % des poissons dans leur site de récolte correspondant, ce qui indique que la majorité des individus de ces sites ne se mêlent pas aux autres. À l’étude de l’ADN microsatellite, il y a des différences génétiques significatives (FST = 0,018 ± 0,004) entre des adultes récoltés à des distances de 340–460 km les uns des autres. En utilisant 5 et 7 locus, il est possible de classer avec succès respectivement 63 % et 75 % des poissons selon leur site de récolte. Ces deux techniques fournissent des informations complémentaires qui corroborent la structure démographique de S. melanops.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: October 1, 2005

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  • Published continuously since 1901 (under various titles), this monthly journal is the primary publishing vehicle for the multidisciplinary field of aquatic sciences. It publishes perspectives (syntheses, critiques, and re-evaluations), discussions (comments and replies), articles, and rapid communications, relating to current research on cells, organisms, populations, ecosystems, or processes that affect aquatic systems. The journal seeks to amplify, modify, question, or redirect accumulated knowledge in the field of fisheries and aquatic science. Occasional supplements are dedicated to single topics or to proceedings of international symposia.
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