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Effect of age and temperature on amino acid composition and the content of different protein types of juvenile Atlantic cod (Gadus morhua) otoliths

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Abstract:

The purpose of this study was to analyse the amino acid composition of otolith matrix protein, estimate the proportion of the water-soluble protein fraction, and analyse the effect of matrix composition on otolith visual appearance. Juvenile Atlantic cod (Gadus morhua) were reared under constant temperature and feeding conditions and sampled at the beginning and the end of the experiment. The amino acid composition was dominated by asparagine, glutamic acid, leucine, serine, and proline. A change in amino acid composition was observed with increasing temperature and time, caused by changing proportions of the water-soluble and -insoluble protein fractions. Feeding level had no effect. The relative content of water-soluble protein was linearly related to fish dry weight and temperature. Otolith opacity, defined as the percentage of incident light absorbed by an otolith section, did not differ significantly between experimental treatments. The soluble protein fraction had a positive, albeit insignificant, correlation with opacity. Using opacity and otolith volume, deposited total otolith protein content was estimated with an R2 of 0.91, where otolith volume alone explained 83% of the observed variation.

L'objectif de notre étude est d'analyser la composition en acides aminés des protéines de la matrice des otolithes, d'estimer la proportion de la fraction protéinique hydro-soluble et d'analyser l'effet de la composition de la matrice sur l'apparence visuelle de l'otolithe. Nous avons élevé de jeunes morues franches (Gadus morhua) dans des conditions constantes de température et d'alimentation et les avons échantillonnées au début et à la fin de l'expérience. La composition en acides aminés est dominée par l'asparagine, l'acide glutamique, la leucine, la sérine et la proline. Il se produit un changement dans la composition des acides aminés en fonction de la température et du temps, qui est causé par une modification des proportions des fractions protéiniques solubles et insolubles dans l'eau. Le niveau d'alimentation est sans effet. Le contenu relatif de protéines hydrosolubles est en relation linéaire avec la masse sèche du poisson et la température. L'opacité de l'otolithe, défini comme le pourcentage de lumière incidente absorbé par une section d'otolithe, ne diffère pas selon les traitements expérimentaux. La fraction de protéines solubles est en corrélation positive, bien que non statistiquement significative, avec l'opacité. À partir de l'opacité et du volume de l'otolithe, nous avons estimé le contenu total de protéines déposées dans l'otolithe avec un R2 de 0,91 et le volume de l'otolithe seul explique 83 % de la variation.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: 2004-06-01

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  • Published continuously since 1901 (under various titles), this monthly journal is the primary publishing vehicle for the multidisciplinary field of aquatic sciences. It publishes perspectives (syntheses, critiques, and re-evaluations), discussions (comments and replies), articles, and rapid communications, relating to current research on cells, organisms, populations, ecosystems, or processes that affect aquatic systems. The journal seeks to amplify, modify, question, or redirect accumulated knowledge in the field of fisheries and aquatic science. Occasional supplements are dedicated to single topics or to proceedings of international symposia.
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