Definitive identification of fatty acid constituents in marine mammal tissues

$50.00 plus tax (Refund Policy)

Buy Article:

Abstract:

Analyses of fatty acid constituents in a sample of bowhead whale (Balaena mysticetus) blubber were conducted using a method that has not been applied before to studies of lipids in marine mammals. It involves creation and analysis of nitrogen (picolinyl) ester derivatives of the fatty acids followed by combined gas chromatography – mass spectrometry for verification of structures. Use of this approach allowed the structural confirmation of 45 different fatty acids in the blubber sample. The traditional method of methyl ester derivatization of fatty acids followed by gas chromatography – mass spectrometry analyses provides more component characterization than analyses by gas chromatography – flame ionization detection, but not enough to effectively differentiate double bond isomers or branched compounds. Although the novel approach is time intensive, we recommend that it be employed for studies where the precise identification and confirmation of fatty acids is important.

L'utilisation d'une méthode qui n'a jamais encore été employée pour étudier les lipides des mammifères marins nous a permis d'analyser les composantes des acides gras dans un échantillon de lard de la baleine franche boréale (Balaena mysticetus). La méthode consiste en la production et l'analyse d'esters azotés (pycolinyliques) dérivés des acides gras, suivies d'une vérification des structures à l'aide d'une combinaison de chromatographie en phase gazeuse et de spectrométrie de masse (GC–MS). Cette méthode a confirmé par analyse des structures la présence de 45 acides gras différents dans l'échantillon de lard. La méthode courante de dérivatisation d'esters méthyliques d'acides gras suivie par des analyses GC–MS produit une meilleure caractérisation des composantes que les analyses par chromatographie en phase gazeuse combinée à une détection par ionisation de flamme (GC–FID), mais elle ne permet pas de différencier de façon concrète les isomères à doubles liens ou les composantes à chaînes latérales. Bien que notre nouvelle méthodologie exige beaucoup de temps, nous en recommandons l'usage dans les études qui requièrent des identifications et des confirmations précises des acides gras.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: April 1, 2004

More about this publication?
  • Published continuously since 1901 (under various titles), this monthly journal is the primary publishing vehicle for the multidisciplinary field of aquatic sciences. It publishes perspectives (syntheses, critiques, and re-evaluations), discussions (comments and replies), articles, and rapid communications, relating to current research on cells, organisms, populations, ecosystems, or processes that affect aquatic systems. The journal seeks to amplify, modify, question, or redirect accumulated knowledge in the field of fisheries and aquatic science. Occasional supplements are dedicated to single topics or to proceedings of international symposia.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more