Fatty acid metabolism in rainbow trout (Oncorhynchus mykiss) tissues: differential incorporation of palmitate and oleate

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Abstract:

Total fatty acid (FA) fluxes of teleost have been well characterized, but the tissues incorporating FA from the circulation and the metabolic fate of these FA remain unclear. Our goals were to determine (i) the relative roles of different tissues in incorporating FA, (ii) the lipid pools that include FA (phospholipids (PL) or neutral lipids (NL)), and (iii) whether saturated and monounsaturated FA behave differently. Fatty acid incorporation was measured after administration of 1-14C-palmitate and 9,10-3H-oleate in rainbow trout (Oncorhynchus mykiss). Results show that FA incorporation varies tremendously among tissues and that the dominant physiological roles of palmitate and oleate are different. For all tissues, oleate is incorporated more rapidly than palmitate into NL (ratio oleate-palmitate 2.8 ± 0.3), showing that oleate plays a prominent role in energy metabolism. However, the two FA enter tissue PL to the same extent (incorporation ratio 1.0 ± 0.2), suggesting equal contributions to membrane turnover. This study reveals a novel aspect of the dominant role played by monounsaturates in energy metabolism: their preferential incorporation in triacylglycerol reserves.

Les flux globaux d'acides gras (FA) sont bien caractérisés chez les téléostéens, mais les tissus où ces acides gras s'incorporent à partir de la circulation et le destin métabolique des acides gras restent encore mal connus. Nos objectifs sont (i) de déterminer les rôles relatifs des divers tissus dans l'incorporation des acides gras, (ii) d'identifier les réservoirs lipidiques dans lesquels les acides gras sont inclus, soit les phospholipides (PL) ou les lipides neutres (NL) et (iii) de voir si les acides gras saturés et monoinsaturés se comportent de façon différente. L'administration de 1-14C-palmitate et de 9,10-3H-oléate à des truites arc-en-ciel (Oncorhynchus mykiss) a permis de mesurer l'incorporation des acides gras. Cette incorporation varie considérablement d'un tissu à l'autre et les rôles physiologiques principaux du palmitate et de l'oléate sont différents. Dans tous les tissus, l'oléate est incorporé plus rapidement dans les lipides neutres que le palmitate (rapport oléate-palmitate 2,8 ± 0,3), ce qui indique que l'oléate joue un rôle primordial dans le métabolisme énergétique. Cependant, les deux acides gras s'incorporent aux phospholipides des tissus au même rythme (rapport des incorporations 1,0 ± 0,2), ce qui indique une contribution égale des deux acides au renouvellement des membranes. Notre étude met en lumière un aspect nouveau du rôle joué par les acides monoinsaturés dans le métabolisme énergétique, soit leur incorporation préférentielle dans les réserves de triacylglycérol.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: October 1, 2003

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  • Published continuously since 1901 (under various titles), this monthly journal is the primary publishing vehicle for the multidisciplinary field of aquatic sciences. It publishes perspectives (syntheses, critiques, and re-evaluations), discussions (comments and replies), articles, and rapid communications, relating to current research on cells, organisms, populations, ecosystems, or processes that affect aquatic systems. The journal seeks to amplify, modify, question, or redirect accumulated knowledge in the field of fisheries and aquatic science. Occasional supplements are dedicated to single topics or to proceedings of international symposia.
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