Red blood cell Hsp 70 mRNA and protein as bio-indicators of temperature stress in the brook trout (Salvelinus fontinalis)

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Abstract:

The purpose of this study was to examine the heat shock protein (Hsp) 70 mRNA and protein response in several tissues of Miramichi brook trout (Salvelinus fontinalis) under both acute and extended heat stress conditions to determine the utility of the red blood cell (rbc) heat shock response as a biomarker of sublethal temperature stress. Red blood cells consistently produced one of the highest responses of all of the tissues examined. Recovery of Hsp 70 mRNA following acute temperature increase required between 24 h and 48 h. In contrast, Hsp 70 protein levels remained significantly elevated for more than 48 h after the heat stress was terminated. During a 6-day extended (23°C) heat stress, rbc Hsp 70 mRNA returned to control levels between 72 and 144 h, whereas Hsp 70 protein was still significantly elevated after 6 days. Thus, although Hsp 70 mRNA proved to be a more sensitive indicator of heat stress in all tissues examined, Hsp 70 protein levels were more sustained. This study confirms the utility of rbcs as a biomarker tissue of thermal stress in fish and indicates that water temperatures presently being reached in brook trout habitats in Canada are capable of inducing a significant heat shock response in this species.

L'objectif de notre étude est d'examiner la protéine de choc thermique (Hsp) 70 ARNm et la réaction protéinique dans plusieurs tissus de l'omble de fontaine de la Miramichi, dans des conditions de stress thermique, tant aiguës que chroniques, dans le but de déterminer l'utilité de la réaction des érythrocytes (rbc) au choc thermique comme bioindicateur du stress thermique sublétal. De tous les tissus examinés, les érythrocytes produisent toujours une des réactions les plus fortes. Le rétablissement de la Hsp 70 ARNm après un accroissement aigu de température prend entre 24 et 48 h. En revanche, les concentrations de protéine Hsp 70 demeurent élevées pour plus de 48 h après la fin du stress thermique. Durant un stress thermique (23 °C) prolongé de 6 jours, la concentration de Hsp 70 ARNm des érythrocytes revient au niveau de celle des témoins en 72 à 144 h, alors que la concentration de protéine Hsp 70 est encore significativement élevée au bout des 6 jours. Ainsi, alors que la Hsp 70 ARNm est un indicateur plus sensible du stress thermique dans tous les tissus examinés, les concentrations de la protéine Hsp 70 se maintiennent plus longtemps. Notre étude confirme l'utilité des érythrocytes comme tissu bioindicateur du stress thermique chez les poissons et démontre que les températures qui sont atteintes actuellement dans les habitats des ombles de fontaine au Canada sont suffisamment élevées pour induire des réactions significatives de choc thermique chez cette espèce.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: April 1, 2003

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  • Published continuously since 1901 (under various titles), this monthly journal is the primary publishing vehicle for the multidisciplinary field of aquatic sciences. It publishes perspectives (syntheses, critiques, and re-evaluations), discussions (comments and replies), articles, and rapid communications, relating to current research on cells, organisms, populations, ecosystems, or processes that affect aquatic systems. The journal seeks to amplify, modify, question, or redirect accumulated knowledge in the field of fisheries and aquatic science. Occasional supplements are dedicated to single topics or to proceedings of international symposia.
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