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Population structure of two ecologically distinct forms of ninespine stickleback, Pungitius pungitius: gene flow regimes and genetic diversity based on mtDNA sequence variations

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The population structure and its effect on genetic diversity were investigated in two ecologically distinct forms (fresh- and brackish-water types) of ninespine stickleback (Pungitius pungitius L.), which coexist on the coast of eastern Hokkaido, Japan. Obvious population subdivision (jST = 0.102 to 0.668) between freshwater systems in the freshwater type compared with that in the brackish-water type (jST = <0 to 0.078) was revealed on the basis of mitochondrial DNA (mtDNA) control region sequence variations. Results of the nested clade analysis suggested that the geographic distribution patterns of mtDNA lineages were mainly due to the contemporary population structure, rather than population histories. Although local populations were sampled from a similar geographic range for the two types, gene and nucleotide diversities of the freshwater type were significantly greater (0.94 and 0.016) than those of the brackish-water type (0.55 and 0.003). The increment of genetic diversity should be attributable to the obvious population structure and existence of divergent mtDNA lineages in the former, which clearly increased the effective population size of a subdivided population. Although these two forms maintain distinct gene pools, small sequence differences among mtDNA haplotypes obtained from each type suggested that mtDNA introgression has occurred between them.

La structure de population et son effet sur la diversité génétique ont pu être étudiés chez deux formes écologiquement distinctes (d'eau douce et d'eau saumâtre) de l'épinoche à neuf épines (Pungitius pungitius L.) qui cohabitent sur la côte orientale d'Hokkaido au Japon. Contrairement à la forme d'eau saumâtre (jST = <0–0,078), il y a chez la forme d'eau douce une nette subdivision de la population (jST = 0,102–0,668) selon les systèmes hydrographiques, mise en évidence par les variations dans les séquences de la région de contrôle de l'ADN mitochondrial (ADNmt). Les résultats d'une analyse emboîtée des clades laisse croire que les patterns de répartition géographique des lignées d'ADNmt sont reliés à la structure contemporaine de la population plutôt qu'au passé de ces populations. Bien que les populations locales des deux formes aient été échantillonnées sur une étendue géographique semblable, la diversité des gènes et celle des nucléotides chez la population d'eau douce sont significativement plus élevées (respectivement, 0,94 et 0,016) que chez la population d'eau saumâtre (0,55 et 0,003). L'accroissement de la diversité génétique doit être attribuable à la structure manifeste de population et l'existence de lignées divergentes d'ADNmt chez la population d'eau douce, ce qui a clairement augmenté la taille effective de cette population subdivisée. Bien que ces deux formes conservent des pools génétiques différents, les faibles différences génétiques observées entre les haplotypes obtenus des deux formes font croire à l'existence d'une introgression de l'ADNmt entre les deux.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: April 1, 2003

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  • Published continuously since 1901 (under various titles), this monthly journal is the primary publishing vehicle for the multidisciplinary field of aquatic sciences. It publishes perspectives (syntheses, critiques, and re-evaluations), discussions (comments and replies), articles, and rapid communications, relating to current research on cells, organisms, populations, ecosystems, or processes that affect aquatic systems. The journal seeks to amplify, modify, question, or redirect accumulated knowledge in the field of fisheries and aquatic science. Occasional supplements are dedicated to single topics or to proceedings of international symposia.
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