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Isolates of endophytic Chaetomium spp. inhibit the fungal pathogen Pyrenophora tritici-repentis in vitro

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Growth of a culturable pathogen, Pyrenophora tritici-repentis (Died.) Drechs., was inhibited by six isolates of endophytic Chaetomium spp. in vitro when grown in apposition. Metabolites of the endophytes, when grown either on agar or in liquid media, also inhibited the pathogen. The culture filtrate of Chaetomium was extracted in 70% ethanol, and the ethanol-soluble fraction that either passed through a molecular sieve of 3000 molecular mass cutoff or was held on 30000 molecular mass cutoff inhibited the pathogen. The high molecular mass fraction was a protein with one major band at 29kDa. The electroeluted band delayed the growth of the pathogen in vitro. Inhibition was retained in filtrate stored frozen, as dried residue, and when rehydrated after drying. Autoclaving reduced, whereas proteinase K treatment did not affect, the activity of the proteinaceous fraction. The data support the hypothesis that endophytic Chaetomium produce a range of metabolites that inhibit P.tritici-repentis in vitro and may affect the pathogen when the fungi co-occur in wheat.

Lorsqu’on les cultive en apposition in vitro, six isolats de l’endophyte Chaetomium spp., on observe l’inhibition de la croissance d’un champignon pathogène cultivable, le Pyrenophora tritici-repentis Died.) Drechs. En culture gélosée ou liquide, les métabolites de l’endophyte inhibent également le pathogène. On a extrait le filtrat de culture du Chaetomium dans l’éthanol à 70%, et la fraction soluble dans l’éthanol, passée à travers un tamis moléculaire avec limite de poids moléculaire de 3000 ou retenue à limite de poids moléculaire de 30000 inhibe le champignon pathogène. La fraction de haut poids moléculaire est une protéine montrant une bande principale à 29kDa. La bande électro-éluée ralentit la croissance du pathogène in vitro. On retient l’inhibition dans le filtrat congelé, dans le résidu séché et avec réhydratation après dessiccation. L’autoclavage réduit, alors qu’un traitement à la protéinase K n’affecte pas l’activité de la fraction protéinique. Les données supportent l’hypothèse que l’endophyte Chaetomium produit une ensemble de métabolites qui inhibent le P. tritici-repentis in vitro, et pourraient affecter le pathogène, lorsque les champignons co-habitent dans le blé.

Document Type: Research Article

Publication date: July 1, 2006

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  • This journal changed title in 2007 and all issues from 2008 onwards can now be found at Botany

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