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Hybridization and introgression between a rare and a common lady’s slipper orchid, Cypripedium candidum and C. parviflorum (Orchidaceae)

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Abstract:

Extinction through hybridization can threaten endangered species when hybrids are vigorous and fertile. We studied hybridization between the endangered small white lady’s slipper (Cypripedium candidum Muhlenberg ex Willdenow) and the common yellow lady’s slipper (Cypripediumparviflorum Salisbury var. makasin (Farwell) Sheviak and C.parviflorum var. pubescens (Willdenow) O.W.Knight) in four Manitoban populations. We used 8 morphological characters and 1061 amplified fragment length polymorphism (AFLP) loci to characterize variation in 182 plants. Canonical correlation analysis indicated that morphology was highly predictive of genetic composition. Ordination analyses of morphological and genetic data, and Bayesian clustering analysis of the genetic data, all clearly distinguished the two parental species. The two varieties of C.parviflorum were morphologically divergent but occupied the same genetic cluster in both ordination and Bayesian analyses. Putative hybrids had considerable morphological overlap with parental species but were mostly genetically intermediate. Five of 79 individuals assigned to C.candidum and 15 of 71 individuals assigned to C.parviflorum had genetic profiles consistent with mixed ancestry. These results suggest that some introgression has occurred, but genetic assimilation of endangered C.candidum by the more common C.parviflorum does not seem imminent. The high local abundance of C.candidum may reduce its susceptibility to gene flow from C.parviflorum.

L'extinction par hybridation peut menacer les espèces en danger lorsque les hybrides sont vigoureux et fertiles. Les auteurs ont étudié l'hybridation entre le petit sabot de la vierge dame blanche (Cypripedium candidum Muhlenberg ex Willdenow) et le sabot de la vierge jaune plus commun (Cypripedium parviflorum Salisbury var. makasin (Farwell) Sheviak et C. parviflorum var. pubescens (Willdenow) O.W.Knight), dans quatre populations du Manitoba. Afin de caractériser la variation chez 182 plantes, ils ont observé 8 caractères morphologiques ainsi que 1061 lieux montrant un polymorphisme de la longueur des fragments de restriction (RFLP). L'analyse des corrélations canoniques indique que la morphologie peut fortement prédire la composition génétique. Les analyses d'ordination des données morphologiques et génétiques ainsi que les analyses de regroupement bayésien des données génétiques permettent de distinguer les deux espèces parentes. Les deux variétés du C. parviflorum divergent morphologiquement, mais occupent le même regroupement, à la fois dans les analyses d'ordination et bayésiennes. Les présumés hybrides montrent un recouvrement morphologique considérable avec les espèces parentes, mais apparaissent généralement intermédiaires génétiquement. Cinq des 79 individus assignés au C. candidum et 15 des 71 individus assignés au C. parviflorum montrent des profiles génétiques congrus avec un mélange ancestral. Ces résultats suggèrent l'existence d'une certaine introgression, mais l'assimilation de l'espèce menacée C. candidum par le C. parviflorum plus abondante, ne semble pas imminente. La forte abondance locale du C. candidum peut réduire sa susceptibilité au flux de gène provenant du C. parviflorum.

Document Type: Research Article

Publication date: November 1, 2009

More about this publication?
  • Published since 1929, this monthly journal features comprehensive research articles and notes in all segments of plant sciences, including cell and molecular biology, ecology, mycology and plant-microbe interactions, phycology, physiology and biochemistry, structure and development, genetics, systematics, and phytogeography. It also publishes commentary and review articles on topics of current interest, contributed by internationally recognized scientists.

    Previously published as the Canadian Journal of Botany
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