Authors: Zawada, Robert J.X.; Kwan, Peggy; Olszewski, Kellen L.; Llinas, Manuel; Huang, Shu-Gui

Source: Biochemistry and Cell Biology, Volume 87, Number 3, June 2009 , pp. 541-544(4)

Publisher: NRC Research Press

Abstract:

Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplate-based, room temperature assay can be applied to determine urea synthesis in cell culture.

L'urée est le principal sous-produit azoté du métabolisme des protéines chez les mammifères. Nous décrivons ici une méthode quantitative sensible de mesure de l'urée à l'aide d'un réactif de Jung modifié. Cet essai est spécifique à l'urée et n'est pas affecté par la présence d'ammoniac, une molécule qui interfère couramment dans les tissus et en culture cellulaire. Nous démontrons que cet essai colorimétrique commode, réalisé en micro-plaques à la température de la pièce, peut être utilisé pour mesurer la synthèse de l'urée dans les cultures cellulaires.

Document Type: Research article

Publication date: 2009-06-01

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• Published since 1929, this bimonthly journal explores every aspect of general biochemistry and includes up-to-date coverage of experimental research into cellular and molecular biology, as well as review articles on topics of current interest and notes contributed by recognized international experts. Special issues each year are dedicated to expanding new areas of research in biochemistry and cell biology.
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