Authors: Ngai P.H.K.; Ng T.B.

Source: Biochemistry and Cell Biology, Volume 81, Number 6, November 2003 , pp. 387-394(8)

Publisher: NRC Research Press

Abstract:

A monomeric protein, with a molecular mass of 25 kDa and an N-terminal sequence resembling a segment of chitin synthase, was isolated from the seeds of the black soybean Glycine soja. The protein, designated glysojanin, demonstrated potent antifungal activity against the fungi Fusarium oxysporum and Mycosphaerella arachidicola. It inhibited HIV-1 reverse transcriptase with an IC₅₀ of 47 µmol/L, [methyl-³H]thymidine incorporation by mouse spleen cells with an IC₅₀ of 175 µmol/L, and translation in the rabbit reticulocyte lysate with an IC₅₀ of 20 µmol/L. Glysojanin was purified using a procedure that involved ion-exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion-exchange chromatography by fast protein liquid chromatography on Mono S, and gel filtration by fast protein liquid chromatography on Superdex 75.Key words: antifungal protein, seeds, soybean, purification.

Une protéine monomérique a été isolée de graines de soja noir Glycine soja. Elle a une masse moléculaire de 25 kDa et une séquence N-terminale semblable à un segment de la chitine synthase. La protéine, nommée glysojanine, a une puissante activité antifongique contre les champignons Fusarium oxysporum et Mycosphaerella arachidicola. Elle inhibe la transcriptase inverse du HIV-1 (IC₅₀ 47 µmol/L), l'incorporation de [méthyl-³H]thymidine dans des cellules de rates de souris (IC₅₀ 175 µmol/L) et la traduction dans un lysat de réticulocytes de lapin (IC₅₀ 20 µmol/L). La glysojanine a été purifiée en utilisant un procédé faisant intervenir une chromatographie d'échange ionique sur DEAE-cellulose, une chromatographie d'affinité sur gel de Affi-gel bleu, une chromatographie d'échange ionique par chromatographie liquide FPLC sur Mono S et une filtration sur gel par chromatographie liquide FPLC sur Superdex 75.Mots clés : protéine antifongique, graines, soja, purification.[Traduit par la Rédaction]

Document Type: Research article

Publication date: 2003-11-01

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• Published since 1929, this bimonthly journal explores every aspect of general biochemistry and includes up-to-date coverage of experimental research into cellular and molecular biology, as well as review articles on topics of current interest and notes contributed by recognized international experts. Special issues each year are dedicated to expanding new areas of research in biochemistry and cell biology.
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