Progesterone and estrogen influence postexercise leukocyte infiltration in overiectomized female rats

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Abstract:

Limited research has been conducted on the effects of progesterone alone, or in combination with estrogen, on leukocyte infiltration in skeletal muscle following exercise. To investigate the effects of these female sex hormones, ovariectomized female rats were divided into 4 exercise and 4 control groups: sham, estrogen, progesterone, and a combination of estrogen plus progesterone. Following 8 days of hormone replacement and 24 h postexercise, soleus (red) and superficial (white) vastus muscles were removed and immunostained for His48 (neutrophil)- and ED1 (macrophage)-positive cells. The postexercise increase in leukocyte infiltration was completely (p < 0.05) attenuated with estrogen supplementation alone in both muscle types, relative to sham. Progesterone treatment alone also resulted in a smaller (20%-30%) but significant (p < 0.05) attenuation of postexercise muscle leukocyte infiltration. The combination of estrogen and progesterone treatment did not significantly alter the attenuation seen with estrogen supplementation alone. Hence, progesterone can independently attenuate postexercise muscle leukocyte infiltration, albeit to a lesser degree than estrogen, and it will not negate or accentuate the effect of estrogen.

Il y a très peu d’études sur les effets de la progestérone seule ou en combinaison avec l’œstrogène sur l’infiltration des leucocytes dans le muscle squelettique après la fin d’un exercice physique. Pour analyser les effets de ces hormones sexuelles féminines, nous avons réparti des rates ovariectomisées dans quatre groupes à l’effort et quatre groupes sans effort : contrôle, œstrogène, progestérone et combinaison d’un traitement d’œstrogène et de progestérone. Après 8 jours d’hormonothérapie substitutive et 24 h après la fin d’un exercice physique, on extrait le muscle soléaire (rouge) et le muscle vaste superficiel (blanc) pour en déterminer par immunocoloration les concentrations de cellules positives : His48 (neutrophile) et ED1 (macrophage). Comparativement à l’observation faite dans le groupe de contrôle, l’augmentation postexercice de l’infiltration des leucocytes est complètement atténuée (p < 0,05) par la supplémentation en œstrogène seul dans les deux types de fibres musculaires. L’administration de progestérone seule suscite une plus faible atténuation (20 à 30 %), mais significative (p < 0,05) de l’infiltration postexercice des leucocytes dans le muscle. La combinaison de l’œstrogène et de la progestérone ne modifie pas significativement l’atténuation observée par l’administration d’œstrogène seul. La progestérone peut donc à elle seule atténuer l’infiltration musculaire des leucocytes après la fin de l’exercice physique, mais à un degré moindre que l’œstrogène et cela n’annulera ni n’augmentera l’effet de l’œstrogène.

Document Type: Research Article

Publication date: December 1, 2008

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  • This bimonthly journal has a 30-year history of publishing, first as the Canadian Journal of Sport Sciences, and later as the Canadian Journal of Applied Physiology. It publishes original research articles, reviews, and commentaries, focussing on the application of physiology, nutrition, and metabolism to the study of human health, physical activity, and fitness. The published research, reviews, and symposia will be of interest to exercise physiologists, physical fitness and exercise rehabilitation specialists, public health and health care professionals, as well as basic and applied physiologists, nutritionists, and biochemists.
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