New primers for promising single-copy genes in fungal phylogenetics and systematics
Abstract:Developing powerful phylogenetic markers is a key concern in fungal phylogenetics. Here we report degenerate primers that amplify the single-copy genes Mcm7 (MS456) and Tsr1 (MS277) across a wide range of Pezizomycotina (Ascomycota). Phylogenetic analyses of 59 taxa belonging to the Eurotiomycetes, Lecanoromycetes, Leotiomycetes, Lichinomycetes and Sordariomycetes, indicate the utility of these loci for fungal phylogenetics at taxonomic levels ranging from genus to class. We also tested the new primers in silico using sequences of Saccharomycotina, Taphrinomycotina and Basidiomycota to predict their potential of amplifying widely across the Fungi. The analyses suggest that the new primers will need no, or only minor sequence modifications to amplify Saccharomycotina, Taphrinomycotina and Basidiomycota.
Document Type: Research Article
Publication date: December 31, 2009
More about this publication?
- Persoonia aims to publish papers dealing with molecular phylogeny and evolution of fungi. A further aim is to promote fungal taxonomy by employing a polythetic approach to clarify the true phylogeny and relationships within the kingdom Fungi. The journal publishes high-quality papers elucidating known and novel fungal taxa at the DNA level, and also strives to present novel insights into evolutionary processes and relationships. Papers to be considered include research articles, topical and book reviews.
Papers are published using a Fast Track system. This implies that the papers are immediately published online and freely available through the internet via this website. For effective publication under the International Code of Botanical Nomenclature the online published papers will be distributed in printed form to several libraries at the same time.
Hard copy volumes: Twice a year (June and December) the online published papers will be bound. These volumes new style will be in A4 format and full colour and will be available through the Publications Department of the National Herbarium of the Netherlands.
Persoonia is a journal published jointly by the Nationaal Herbarium Nederland and the Centraalbureau voor Schimmelcultures.
Fast Track articles are available here
If there are no articles shown in this section, all the Fast Track articles are published in the latest issue of Persoonia.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- ingentaconnect is not responsible for the content or availability of external websites