HLA-DR and -DQ polymorphism in Cameroon

Authors: Pimtanothai N.¹; Hurley C.K.¹; Leke R.²; Klitz W.³; Johnson A.H.⁴

Source: Tissue Antigens, Volume 58, Number 1, July 2001 , pp. 1-8(8)

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Abstract:

Abstract:

HLA-DRB1, -DQA1 and -DQB1 allele frequencies were determined by high-resolution polymerase chain reaction using sequence-specific oligonucleotide probes (PCR-SSOP) and/or DNA sequencing in 126 healthy individuals in Cameroon. Eighteen DRB1, 11 DQA1, and 18 DQB1 alleles were observed. The most common alleles at each locus were DRB1*1503 (29%), DRB1*1301 (13%); DQA1*0102 (38%), DQA1*0302 (11%), DQA1*0401 (11%); and DQB1*0602 (31%), DQB1*0301 (13%), DQB1*0501 (12%). Forty-four different haplotypes were identified including 12 novel haplotypes demonstrating the HLA class II diversity resulting from allele combinations in this population. A single predominant DRB1*1503-DQA1*0102-DQB1*0602 haplotype was observed with a frequency of 27%. In summary, this study of HLA class II polymorphism in Cameroon demonstrates the extent of diversity in this population.

Keywords: Cameroon; HLA-DR, DQ; HLA haplotype; PCR-SSOP; polymorphism

Language: English

Document Type: Original article

DOI: http://dx.doi.org/10.1034/j.1399-0039.2001.580101.x

Affiliations: 1: Department of Microbiology and Immunology, Georgetown University Medical Center, Washington, D.C., USA, 2: University of Yaounde I, Yaounde, Cameroon, 3: School of Public Health, University of California, Berkeley, California, USA 4: Department of Pediatrics,Georgetown University Medical Center, Washington, D.C., USA, *

Publication date: 2001-07-01