Skip to main content

Wavelength effects on contrast observed with reflectance in vivo confocal laser scanning microscopy

Buy Article:

$43.00 plus tax (Refund Policy)


The ability to optically section live biological tissue in vivo with laser light is made possible by confocal laser scanning microscopy (CLSM). In this work, the effects of changing the wavelength of incident light used for CLSM imaging of human skin are reported and analyzed. Methods:

Optical phantoms and the skin of eight human volunteers were imaged using CLSM systems having three different incident light wavelengths (405, 785, and 830 nm). Results:

Qualitative and quantitative differences were observed between images obtained at each wavelength, despite the proximity of the two near infrared 785 and 830 nm wavelengths. Furthermore, the penetration depth achieved with the 405 nm CLSM permitted imaging into the papillary dermis. Conclusion:

The laser wavelength used in CLSM reflectance imaging is important to properly understand and resolve different biological structures within human skin.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: confocal laser scanning microscopy; dermatology; medical imaging; non-invasive imaging; reflectance confocal microscopy; scanning confocal laser microscopy; scanning laser confocal microscopy; wavelength

Document Type: Research Article

Affiliations: 1: Johnson & Johnson Consumer and Personal Products Worldwide, Skillman, NJ, USA and 2: School of Biomedical Engineering, Science & Health Systems, Drexel University, Philadelphia, PA, USA

Publication date: 2009-11-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more