Skip to main content

Inactivation of α1-Antiproteinase Induced by Phenylbutazone: Participation of Peroxyl Radicals and Hydroperoxide

Buy Article:

$51.00 plus tax (Refund Policy)


To clarify the action of a side-effect of phenylbutazone, we investigated the inactivation of α1-antiproteinase induced by phenylbutazone in the presence of horseradish peroxidase (HRP) and H2O2 (HRP-H2O2). The activity of α1-antiproteinase was rapidly lost during the interaction of phenylbutazone with HRP-H2O2 under aerobic conditions. Phenylbutazone showed a marked spectral change under aerobic conditions but not under anaerobic conditions. Spin trap agents were very effective in inhibiting α1-antiproteinase inactivation induced by phenylbutazone. Oxidation of phenylbutazone was stopped by catalase, but the inactivation reaction of α1-antiproteinase proceeded even after removal of H2O2 in the reaction mixture. Formation of the peroxidative product from phenylbutazone was detected by iodometric assay. These results indicate that both peroxyl radicals and the peroxidative product of phenylbutazone participated in the inactivation of α1-antiproteinase. Other anti-inflammatory drugs did not inactivate α1-antiproteinase during interaction with HRP-H2O2. Inactivation of α1-antiproteinase may contribute to serious side effects of phenylbutazone.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Document Type: Research Article

Affiliations: Hokkaido Pharmaceutical University, Katsuraoka-cho 7-1, Otaru 047-0264, Japan

Publication date: 2006-09-01

More about this publication?
  • Formerly Pharmacology & Toxicology
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more