Skip to main content

Water-Soluble Polysaccharide from Eleutherococcus senticosus Stems Attenuates Fulminant Hepatic Failure Induced by D-Galactosamine and Lipopolysaccharide in Mice

Buy Article:

$51.00 plus tax (Refund Policy)

Abstract:

Abstract:

The aim of this study was to investigate whether Eleutherococcus senticosus stems could attenuate D-galactosamine/lipopolysaccharide-induced fulminant hepatic failure in mice. E. senticosus, known as Siberian ginseng, is a popular folk medicine used as a tonic in Asia. Preparations of E. senticosus used in this study were as follows; (i) 70% ethanol extract (ii) water extract (iii) ethanol-soluble part of the water extract (iv) polysaccharide obtained as an 80% ethanol insoluble of the water extract. Preparations were given by intraperitoneal (300 mg/kg and 50 mg/kg) or oral (300 mg/kg) injection at 12 hr and 1 hr before a D-galactosamine/lipopolysaccharide injection. The intraperitoneal injection of water extract and polysaccharide significantly lowered serum levels of tumour necrosis factor-α, aspartate transaminase and alanine transaminase, improved the histologic changes in liver, inhibited hepatocyte apoptosis confirmed by the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling method and DNA fragmentation assay, and suppressed the lethality induced by D-galactosamine/lipopolysaccharide. The oral administration of water extract and polysaccharide also reduced serum aspartate transaminase, alanine transaminase and tumour necrosis factor-α levels. In contrast 70% ethanol extract and ethanol-soluble part of the water extract had no protective effect when treated intraperitoneally or orally. These results indicate E. senticosus stems attenuate fulminant hepatic failure induced by D-galactosamine/lipopolysaccharide in mice and the protective effect is due to water-soluble polysaccharides in E. senticosus stems.

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1742-7843.2004.pto940607.x

Affiliations: 1: Department of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea 2: College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea 3: Anticancer Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, P.O.Box 115, Yuseong, Daejeon 305-600, Republic of Korea

Publication date: June 1, 2004

More about this publication?
  • Formerly Pharmacology & Toxicology
mksg/pto/2004/00000094/00000006/art00007
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more