Skip to main content

‐activated synthesis of metalloproteinases 1, 3 and 9 is prevented by a broad‐spectrum sunscreen

Buy Article:

$51.00 plus tax (Refund Policy)


Specific sunscreens against ultraviolet (UV) A and B radiations are essential to prevent matrix degradation and the activation of intracellular signaling pathways involved in photoaging and photocarcinogenesis. Matrix degradation results from UVA‐induced production of matrix metalloproteinases (MMP) and activation of intracellular pathways in fibroblasts and keratinocytes. In particular, in keratinocytes, UVA radiation induces β‐catenin nuclear translocation and stimulates MMP gene transcription. Our study was aimed at assessing the efficacy of a specific broad‐spectrum sunscreen in preventing β‐catenin translocation and MMPs enhanced expression in cultured keratinocytes after UVA irradiation.

Sunscreen or the vehicle was spread on quartz sheet. Irradiation of HaCaT cells with 6 J/cm2 UVA was performed through the sheet, and cells were collected for β‐catenin immunostaining then visualization by confocal microscopy, and quantitative real‐time polymerase chain reaction analysis of MMP‐1, ‐3 and ‐9 gene expression.

As shown by immunostaining and confocal microscopy, the sunscreen abrogated UVA‐induced beta‐catenin translocation to the nucleus, in comparison with control groups. MMP‐1, ‐3 and ‐9 mRNA expression was enhanced by 7, 7 and 4 folds (P < 0.0001, P < 0.001 and P < 0.01, respectively) in unprotected UVA‐irradiated cells compared to the non‐irradiated control. Sunscreen protection of the cells significantly reduced UVA‐induced expression of MMP‐1, ‐3 and ‐9 by 83% (P < 0.01), 80% (P < 0.01) and 65% (P < 0.05), respectively.

This study demonstrated the efficacy of this broad‐spectrum sunscreen in preventing UVA‐induced effects on the markers of photoaging and photocarcinogenesis in vitro. It was able to protect HaCaT keratinocytes from UVA‐induced β‐catenin translocation to the nucleus and MMPs expression.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Document Type: Research Article

Publication date: 2011-12-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more