Protective effect of the Baicalin against DNA damage induced by ultraviolet B irradiation to mouse epidermis
To investigate whether topical application of Baicalin affords protecting Balb/C mice epidermis from ultraviolet (UV)B-induced DNA damage and its underlying mechanisms. Methods:
A DNA damage model of UVB irradiation-induced mice epidermis was established. The immunohistochemical staining, Southwestern dot-blotting were used for cyclobutane pyrimidine dimers (CPDs) detection; Western blotting was used for p53 detection; reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA level of Bcl-2 and Bax; terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) was used to detect apoptotic cells. Results:
Topical application of Baicalin on Balb/C mice skin significantly decreased the amount of epidermal CPDs 1, 24 and 48 h after 180 mJ/cm2 of UVB irradiation as compared with untreated mice. UVB-induced apoptosis was less pronounced in Baicalin-treated mice epidermis, which was accompanied by less p53 accumulation and higher Bcl-2/Bax ratio compared with that of untreated mice. Conclusion:
Taken together, these results suggest that topical Baicalin application mitigates DNA photo-damage. Baicalin is therefore a promising protective substance against UVB radiation.
Document Type: Research Article
Affiliations: Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Publication date: August 1, 2008