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The effect of psoralen plus ultraviolet A in vitro in HUT-78 enhances by 5-aminolevulinic acid

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Sezary syndrome and mycosis fungoides are forms of cutaneous T-cell lymphoma, and in the early stage of these diseases psoralen plus ultraviolet A (PUVA) is one of the treatments of choice. Photodynamic therapy using 5-aminolevulinic acid (ALA-PDT) is an effective, non-invasive, and safe treatment for most superficial skin cancers. In order to obtain greater efficacy of PUVA, we investigated the synergistic anti-tumor effects of ALA-PDT and PUVA using 8-methoxypsoralen (8-MOP) and a UVA lamp. Methods:

The in vitro effects of PUVA and ALA-PDT and their combination in HUT-78 cell line from human SS were determined by MTT assay. Results:

In our results, cell proliferation compared with controls was inhibited to 53.2% with UVA alone, 52.3% with 1 M 8-MOP, 43.8% with 100 M ALA, and 19.2% with combined 8-MOP and ALA. Conclusion:

Combined use of ALA and PUVA using 8-MOP and UVA lamps, which are widespread in Japan, had a strong anti-tumor effect in vitro. Combined treatment with ALA-PDT and PUVA using a UVA lamp appears to have a strong treatment effect.

Keywords: 5-aminolevulinic acid; cutaneous T cell lymphoma; photodynamic therapy; protoporphyrin IX; psoralen plus ultraviolet A

Document Type: Research Article


Affiliations: Department of Dermatology, Aichi Medical University School of Medicine, Nagakute, Japan

Publication date: 2007-04-01

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