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Whole-body UVB (TL-01) or UVA-1 irradiation does not alter the levels of immunomodulatory cytokines in the serum of human volunteers

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Abstract:

Background/Purpose:

Ultraviolet (UV) exposure of mammalian skin induces local and systemic immunosuppression. In mice it has been proposed that systemic immunosuppression is mediated by an UV-induced cytokine cascade involving systemic interleukin (IL)-4 and IL-10 and a reduction in IL-12 activity. To investigate whether there was a parallel mechanism in humans we examined the effect of whole-body narrowband ultraviolet B (UVB) (311–313 nm; TL-01) and ultraviolet A (UVA)-1 (340–400 nm) on serum cytokine levels. Methods/Results:

In a first study, five male psoriatic subjects were whole-body irradiated with three sessions of a standard UVB (TL-01) phototherapy regimen previously shown to cause downregulation of natural killer cell activity and T helper 1 (Th1) and Th2 cytokine production by peripheral blood mononuclear cells. Enzyme-linked immunoabsorbent assay (ELISA) of sera taken before and after the third session showed no effect of phototherapy on IL-10 and tumour necrosis factor-α (TNF-α). In a second study, five healthy subjects received three whole-body exposures of UVB (TL-01) and five other healthy subjects received three exposures of UVA-1 on alternate days (total 22 J/cm2). Blood samples were taken before the first irradiation and at 0, 4, 8, 12, 14, 24 and 48 h after the third irradiation. The sera were subsequently analysed for IL-10, IL-12, IL-8, IL-1 and TNF-α, by ELISA. The levels of IL-1 and TNF-α were below detection limits (<5 pg/ml), while no significant change in the levels of IL-10, IL-12 or IL-8 was detected as a result of either TL-01 or UVA-1. Conclusion:

It seems unlikely that a modulation in these circulating cytokines assessed in this study accounts for systemic UV-induced immunosuppression in human subjects.

Keywords: UV-induced immunosuppression; cytokines; whole-body UV irradiation

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1600-0781.2004.00089.x

Affiliations: 1: Photobiology Unit, Ninewells Hospital and Medical School, Dundee, UK, 2: RIVM, Bilthoven, The Netherlands, 3: Medical Microbiology, University of Edinburgh, UK, and 4: Photobiology Unit, Dermatology Sciences, University of Manchester, Hope Hospital, Manchester, UK

Publication date: April 1, 2004

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