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Effect of the spectral range of a UV lamp on the production of cyclobutane pyrimidine dimers in human skin in situ

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Abstract:

Background:

Ultraviolet (UV) irradiation has a broad spectrum of biological effects and a capacity to initiate skin carcinogenesis through DNA damage. The effect of different wave bands of UV light on the production of DNA damage in human skin in situ was studied with a broadband UV-B lamp TL-12 and a narrowband UV-B lamp TL-01. Methods:

Eight psoriasis patients participated in the study. Their minimal erythema dose was assessed separately for the two UV-B wave band ranges. Test areas of buttock skin were irradiated with the two spectrally differing lamps using erythemally equivalent UV doses of 40 and 80 mJ/cm2 CIE (Commission International de I'Eclairage). Punch biopsies were taken from the irradiated areas, and UV-induced DNA lesions (cyclobutane pyrimidine dimers, CPDs) in the skin were analyzed with a 32P high-performance liquid chromatography postlabelling method. Results:

No UV source-dependent differences in the induced levels of CPDs were detected in this study. Conclusion:

CPD production with broadband TL-12 and narrowband TL-01 UV-B lamps in situ did not differ when erythemally equivalent UV doses were used. The preliminary result needs to be confirmed in a larger study.

Keywords: CPD; UV radiation; broadband; narrowband; nucleotide excision repair

Document Type: Research Article

DOI: http://dx.doi.org/10.1046/j.1600-0781.2003.00063.x

Affiliations: 1: Department of Dermatology, Päijät-Häme Central Hospital, Lahti, Finland, 2: Department of Biosciences, Novum, Karolinska Institute, Huddinge, Sweden, and

Publication date: December 1, 2003

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