Effect of UV on the susceptibility of acid-soluble Skh-1 hairless mouse collagen to collagenase
Methods: Acid- extracted Skh-1 hairless mouse collagen samples were irradiated with 0–140 J/cm2 of radiation from bank of filtered FS lamp (UVB/UVA = 0.33, fluence rate = 0.81 mW/cm2). Subsequent to UV irradiation, collagen samples were coupled with fluorescein isothiocyanate (FITC) and assayed for susceptibility to bacterial collagenase by monitoring the appearance of supernatant FITC fluorescence (a measure of lysed collagen) over time of incubation. As a ‘reference’, unirradiated commercial FITC-labelled citrate-soluble collagen (Elastin Products, Owensville, MO 65066, USA) was similarly analysed.
Results: Unirradiated mouse collagen had a lower rate of cleavage than did the calfskin sample. Irradiation of unlabelled mouse collagen for 0–48 h (0–140 J/cm2 total UV) rendered the sample more soluble, with concomitant chain degradation, cross-linking and loss of intrinsic collagen fluorescence. At irradiation time's ≥ 4 h (≥11.7 J/cm2), the irradiated collagen was significantly more susceptible to bacterial collagenase digestion.
Discussion: It appears that the rate of cleavage depends on the superstructure of the collagen, since the kinetics of collagen cleavage differ for two collagen samples having essentially the same primary structure. Cleavage kinetics may depend on the ‘maturity’ (solubility) of the collagen. The observation that UV-damaged mouse collagen is a better substrate for collagenase than the intact sample may be illustrative of a mechanism whereby damaged collagen targets itself for selective attack by collagenase.
Document Type: Research Article
Affiliations: 1: Department of Medicine, Morehouse School of Medicine, Atlanta, GA, USA, 2: Division of Dermatology, Department of Medicine, University of Tennessee Center for the Health Sciences, Memphis, TN, USA,
Publication date: 2003-02-01