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Photochemotherapy of vascular cells with 8-methoxypsoralen and visible light: differential effects on endothelial and smooth muscle cells

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Abstract:

Background:

The long-term efficacy of percutaneous transluminal coronary angioplasty is limited by the restenosis which occurs in approximately 40% of patients, usually within 6 months of the procedure. Purpose:

The present study was designed to evaluate the effects of 8-methoxypsoralen (8-MOP) activated with visible light on the properties of bovine aortic smooth muscle cells (SMC) and endothelial cells (EC) in vitro. Methods:

Cells were seeded in polystyrene wells, allowed to attach over a 24-h period, incubated with 1, 20, or 50 µg/ml 8-MOP and then exposed to 12 J/cm2 visible light (447 nm). Cell counts were performed for up 14 days (n = 4–6 wells per time point), and each experiment was performed in triplicate. Cellular migration, morphology, and size were also analyzed. Results:

The lowest 8-MOP dose (1 µg/ml) had no significant effect on SMC proliferation, while the highest dose (50 µg/ml) induced cytostasis. An intermediate dose of 8-MOP (20 µg/ml) produced a transient and reversible inhibition of proliferation. There was no significant effect on proliferation of EC at lowest dose of 8-MOP (1 µg/ml). However, in contrast to the SMC experiments, a transient and reversible inhibition of EC proliferation was seen at both 20 and 50 µg/ml 8-MOP. Conclusions:

These experiments demonstrate that while 8-MOP photoactivated with 447 nm visible light can reversibly inhibit the proliferation of both SMC and EC in a dose-dependent fashion, SMC are more sensitive to the treatment than EC.

Keywords: 8-MOP; phototherapy; vascular cells

Document Type: Research Article

DOI: http://dx.doi.org/10.1034/j.1600-0781.2002.02770.x

Affiliations: Department of Surgery (Vascular), Yale University School of Medicine, New Haven, CT 06510, USA

Publication date: October 1, 2002

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